IgH somatic hyper mutation analysis of spleen B cells of HIS (BALB- Rag/γ) mice
| B cells . | Total unique sequences . | Mutated sequencesa . | % of mutation frequencyb . | Mutation range . |
|---|---|---|---|---|
| Donor 1 IgD+CD27− | 20 | 0 | – | 0–2 |
| Donor 1 IgD+CD27+ | 15 | 10 (67%) | 2.6 | 3–14 |
| Donor 2 IgD+CD27+ | 18 | 8 (44%) | 3.1 | 3–16 |
| Donor 3 IgD+CD27+ | 10 | 4 (40%) | 4.5 | 4–18 |
| B cells . | Total unique sequences . | Mutated sequencesa . | % of mutation frequencyb . | Mutation range . |
|---|---|---|---|---|
| Donor 1 IgD+CD27− | 20 | 0 | – | 0–2 |
| Donor 1 IgD+CD27+ | 15 | 10 (67%) | 2.6 | 3–14 |
| Donor 2 IgD+CD27+ | 18 | 8 (44%) | 3.1 | 3–16 |
| Donor 3 IgD+CD27+ | 10 | 4 (40%) | 4.5 | 4–18 |
Only unique rearrangements were considered for analysis. Sequences scored as mutated contained three or more mutations (≥1% mutation frequency). In brackets is the frequency of mutated sequences among all sequences.
The percentage of point mutations among the total number of sequenced nucleotides of mutated sequences.