IgH SHM analysis of fetal spleen B cells
| B cells . | Total unique sequences . | Mutated sequencesa . | % of mutation frequencyb . | Mutation range . |
|---|---|---|---|---|
| Donor 1 IgD+CD27− | 11 | 0 | – | – |
| Donor 1 IgD+CD27+ | 18 | 3 (17%) | 2.3 | 5–7 |
| Donor 2 IgD+CD27+ | 19 | 3 (16%) | 2.7 | 5–11 |
| Donor 3 IgD+CD27+ | 29 | 6 (21%) | 2.4 | 3–12 |
| B cells . | Total unique sequences . | Mutated sequencesa . | % of mutation frequencyb . | Mutation range . |
|---|---|---|---|---|
| Donor 1 IgD+CD27− | 11 | 0 | – | – |
| Donor 1 IgD+CD27+ | 18 | 3 (17%) | 2.3 | 5–7 |
| Donor 2 IgD+CD27+ | 19 | 3 (16%) | 2.7 | 5–11 |
| Donor 3 IgD+CD27+ | 29 | 6 (21%) | 2.4 | 3–12 |
Only unique rearrangements were considered for analysis. Sequences scored as mutated contained three or more mutations (≥1% mutation frequency). In brackets is the frequency of mutated sequences among all sequences.
The percentage of point mutations among the total number of sequenced nucleotides of mutated sequences.