Mutation frequency at various Ig and non-Ig loci in BL2 clones transfected with WT-AID-EGFP or mutNES-AID-EGFP inducible expression vectors
. | WT-24 clone . | mutNES-16 clone . | |
|---|---|---|---|
| . | number of mutations/total number of sequences (mutation frequency) . | . | |
| Gene locus (domain sequenced) . | . | . | |
| V4-39 (390 bp) | 13/65 (0.51 × 10−3) | 69/74 (2.39 × 10−3) | |
| Cmu (403 bp) | 5/74 (0.17 × 10−3) | 6/73 (0.20 × 10−3) | |
| BCL6 (640 bp) | 3/47 (0.10 × 10−3) | 12/51 (0.37 × 10−3) | |
| TP53 (1,064 bp) | 1/39 (0.024 × 10−3) | 14/43 (0.31 × 10−3) | |
| PAX5 (885 bp) | 6/47 (0.14 × 10−3) | 8/46 (0.20 × 10−3) | |
| AID cDNA (594 bp) | 14/22 (1.07 × 10−3) | 39/22 (2.98 × 10−3) | |
. | WT-24 clone . | mutNES-16 clone . | |
|---|---|---|---|
| . | number of mutations/total number of sequences (mutation frequency) . | . | |
| Gene locus (domain sequenced) . | . | . | |
| V4-39 (390 bp) | 13/65 (0.51 × 10−3) | 69/74 (2.39 × 10−3) | |
| Cmu (403 bp) | 5/74 (0.17 × 10−3) | 6/73 (0.20 × 10−3) | |
| BCL6 (640 bp) | 3/47 (0.10 × 10−3) | 12/51 (0.37 × 10−3) | |
| TP53 (1,064 bp) | 1/39 (0.024 × 10−3) | 14/43 (0.31 × 10−3) | |
| PAX5 (885 bp) | 6/47 (0.14 × 10−3) | 8/46 (0.20 × 10−3) | |
| AID cDNA (594 bp) | 14/22 (1.07 × 10−3) | 39/22 (2.98 × 10−3) | |