Table I.

Transgenic mice that express the apoL-I gene ameliorate infection with T. brucei, T. evansi, and T. congolense

Transgenic DNA
Trypanosome species (1.78 × 106 injected i.p.)
Number mice alive/ number mice infected

 

 
Day 3
 
Day 20
 
Day 50
 
ApoL-I T. brucei  5/5 3/5b 
    Vector  0/5a   
ApoL-I T. brucei SRA 0/5a   
    Vector  0/5a   
ApoL-I T. evansi  5/5 4/5c 
    Vector   5/5 1/5d 
ApoL-I T. congolense  5/5 3/5b 
    Vector   2/5 2/5d 
Transgenic DNA
Trypanosome species (1.78 × 106 injected i.p.)
Number mice alive/ number mice infected

 

 
Day 3
 
Day 20
 
Day 50
 
ApoL-I T. brucei  5/5 3/5b 
    Vector  0/5a   
ApoL-I T. brucei SRA 0/5a   
    Vector  0/5a   
ApoL-I T. evansi  5/5 4/5c 
    Vector   5/5 1/5d 
ApoL-I T. congolense  5/5 3/5b 
    Vector   2/5 2/5d 

Mice expressing the gene for apoL-I were infected with 1.78 × 106T. b. brucei (Lister 427-derived line), T. b. brucei-SRA (Lister 427-derived line expressing the SRA gene), T. evansi (AnTat 3), or T. congolense (STIB 68Q). n = 5 per group.

a

Parasitemia ≥ 1 × 109 before mice were killed.

b

No parasites detectable.

c

Died of unknown cause, did not have detectable parasites.

d

Parasitemia = 1 × 106/ ml.

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