Table I.

Selected proteins containing APIM

Type/group of proteins Proteins Source 
Proteins containing PIP box
and APIM 
DNA ligase I, MDN1 Midasin, ubiquitin thioesterase FAF-X,
protein 18 homologue (hVPS18), cytokine signaling 6 (SOCS-6),
Topo II β, IκB-related protein, UHRF2, PARP4 
Moldovan et al., 2007  
DNA polymerase Pol ζ catalytic subunit (hREV3L) Moldovan et al., 2007  
DNA ligases DNA ligase I, DNA ligase IV Moldovan et al., 2007  
Topo Topo II α and β This study; Niimi et al., 2001;
Lou et al., 2005  
DNA repair proteins hABH2, PARP-1, -2, and -4, RAD51B, FANCC, XPA This study;
Simbulan-Rosenthal et al., 1999;
Jacquemont and Taniguchi, 2007  
DNA repair–associated/
interacting proteins 
XPA-binding protein 2, BRCA1/BRCA2-containing
complex subunit 45 (prot-BRE), x-ray radiation
resistance-associated protein 1 
NA 
Sister chromatid cohesion N-acetyltransferase ESCO1/EFO1, hSMC5 Potts et al., 2006;
Moldovan et al., 2007  
Chromatin remodelling and
DNA-binding proteins 
Chromodomain helicase DNA-binding proteins 3–5,
p325 subunit of remodeling and spacing factor
chromatin–remodelling complex, telomeric repeat–binding
protein 2 (TRF2) 
Opresko et al., 2004  
E3 ubiquitin ligases UHFR1, UHFR2, UBR1, UBR2, ring finger proteins 3, 17,
and 151, probable E3 ubiquitin protein ligase MYCBP2 
Bronner et al., 2007  
Transcription factors TFIIS-L, TFII-I, TFIIE-α, sterol regulatory element-binding
transcription factor 2 (SREBF2), TFIIIC subunit α, TFIID 100 kD
subunit (TAF5), TFIIIC 102 kD subunit (TF3C γ), transcription
factor–like protein MRG15 and X (mortality factor 4–like proteins
1 and 2), E2 transcription factor 7 
This study 
Cell cycle regulators Cell division cycle-associated 2, Bcl2-interacting mediator
of cell death, testis spermatocyte apoptosis-related gene 2 protein 
NA 
Protein kinases Serine/threonine (S/T) protein kinases SRPK1 and -2, 33
and MST4, leucine-rich repeat S/T protein kinase 1, STK23
(S/T protein kinase 23), S/T protein kinase PLK3,
microtubuli-associated S/T protein kinase, microtubuli-associated
S/T protein kinase 1, MAPKAP kinase 2 (MK2) and 5 (MK5),
mitogen-activated protein kinase 15 (MSK-15) 
NA 
Methyltransferase H3 lysine 4–specific MLL3, H3-K9 methyltransferase 5,
putative rRNA methyltransferase 3 
NA 
Cancer-associated antigens Melanoma-associated antigen E1 (MAGE E1), MAGE B18,
MAGE-G1, natural killer tumor recognition protein (NK-TR),
Myc-binding protein–associated protein, Myb-binding
protein 1A, hepatoma-derived growth factor–related protein 2
isoform1, serologically defined colon cancer antigen 1 
NA 
RNA polymerase and
ribosome-associated
proteins 
RNA polymerase II, largest subunit (RPB1), RNA polymerase III
subunit 5 (RPC5), RNA polymerase II 140 kD (RPB2), UTP14A U3
small nucleolar RNA-associated protein 14 homologue A, 60S ribosomal protein L18, 60S ribosomal protein L35,
TAF5-like RNA polymerase II p300 (PAF65-beta), mediator of
RNA polymerase II
transcription subunit 12 homologue 
NA 
Type/group of proteins Proteins Source 
Proteins containing PIP box
and APIM 
DNA ligase I, MDN1 Midasin, ubiquitin thioesterase FAF-X,
protein 18 homologue (hVPS18), cytokine signaling 6 (SOCS-6),
Topo II β, IκB-related protein, UHRF2, PARP4 
Moldovan et al., 2007  
DNA polymerase Pol ζ catalytic subunit (hREV3L) Moldovan et al., 2007  
DNA ligases DNA ligase I, DNA ligase IV Moldovan et al., 2007  
Topo Topo II α and β This study; Niimi et al., 2001;
Lou et al., 2005  
DNA repair proteins hABH2, PARP-1, -2, and -4, RAD51B, FANCC, XPA This study;
Simbulan-Rosenthal et al., 1999;
Jacquemont and Taniguchi, 2007  
DNA repair–associated/
interacting proteins 
XPA-binding protein 2, BRCA1/BRCA2-containing
complex subunit 45 (prot-BRE), x-ray radiation
resistance-associated protein 1 
NA 
Sister chromatid cohesion N-acetyltransferase ESCO1/EFO1, hSMC5 Potts et al., 2006;
Moldovan et al., 2007  
Chromatin remodelling and
DNA-binding proteins 
Chromodomain helicase DNA-binding proteins 3–5,
p325 subunit of remodeling and spacing factor
chromatin–remodelling complex, telomeric repeat–binding
protein 2 (TRF2) 
Opresko et al., 2004  
E3 ubiquitin ligases UHFR1, UHFR2, UBR1, UBR2, ring finger proteins 3, 17,
and 151, probable E3 ubiquitin protein ligase MYCBP2 
Bronner et al., 2007  
Transcription factors TFIIS-L, TFII-I, TFIIE-α, sterol regulatory element-binding
transcription factor 2 (SREBF2), TFIIIC subunit α, TFIID 100 kD
subunit (TAF5), TFIIIC 102 kD subunit (TF3C γ), transcription
factor–like protein MRG15 and X (mortality factor 4–like proteins
1 and 2), E2 transcription factor 7 
This study 
Cell cycle regulators Cell division cycle-associated 2, Bcl2-interacting mediator
of cell death, testis spermatocyte apoptosis-related gene 2 protein 
NA 
Protein kinases Serine/threonine (S/T) protein kinases SRPK1 and -2, 33
and MST4, leucine-rich repeat S/T protein kinase 1, STK23
(S/T protein kinase 23), S/T protein kinase PLK3,
microtubuli-associated S/T protein kinase, microtubuli-associated
S/T protein kinase 1, MAPKAP kinase 2 (MK2) and 5 (MK5),
mitogen-activated protein kinase 15 (MSK-15) 
NA 
Methyltransferase H3 lysine 4–specific MLL3, H3-K9 methyltransferase 5,
putative rRNA methyltransferase 3 
NA 
Cancer-associated antigens Melanoma-associated antigen E1 (MAGE E1), MAGE B18,
MAGE-G1, natural killer tumor recognition protein (NK-TR),
Myc-binding protein–associated protein, Myb-binding
protein 1A, hepatoma-derived growth factor–related protein 2
isoform1, serologically defined colon cancer antigen 1 
NA 
RNA polymerase and
ribosome-associated
proteins 
RNA polymerase II, largest subunit (RPB1), RNA polymerase III
subunit 5 (RPC5), RNA polymerase II 140 kD (RPB2), UTP14A U3
small nucleolar RNA-associated protein 14 homologue A, 60S ribosomal protein L18, 60S ribosomal protein L35,
TAF5-like RNA polymerase II p300 (PAF65-beta), mediator of
RNA polymerase II
transcription subunit 12 homologue 
NA 

NA, not applicable. Bold indicates proteins localized in replication foci under normal conditions or after DNA damage. The full lists of hits for the APIM and PIP motifs are available at http://tare.medisin.ntnu.no/pcna/index.php.

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