Table I.

Mutations in CDC28 suppress GCR rates

Relevant genotype Mutation rate (Canr − 5FOAr) (×10−10
 CDC28 cdc28-as1 cdc28-5M 
WT 3.50 (1)a <12.5 (<3.6)b <128 (<37)b 
CKIs    
sic1Δ 59.5 (17) NT NT 
far1Δ 71.1 (20) NT NT 
sic1Δ far1Δ 115.9 (33) NT NT 
swe1Δ <5.69 (<1.6)b NT NT 
HR    
rad50Δ 2,300 (657)a 148 (42) NT 
mre11Δ 2,490 (711) 128 (37) <327 (93)b 
mre11Δ in nocodazoleg 5,700 (1,622) NT NT 
mre11Δ in SC, RTh 1,890 (543) NT NT 
mre11Δ yku80Δ 6,156 (1,759) 542 (155) NT 
rad52Δ 350 (100)c 290 (83) <446 (127)b 
sae2Δ 177 (51) <67 (<19)b NT 
sae2Δ mre11Δ 2,798 (797) NT NT 
DNA damage and replication checkpoint    
mec1Δ 680 (194)d NT <33 (<9)b 
mec1Δ tel1Δ 45,000 (12,857)d NT 2,390 (683) 
rad53Δ 95 (27)d <9.6 (<2.7)b NT 
pds1Δ 670 (190)d <64 (<18)b NT 
rfc5-1Δ 660 (189)d <5.1 (<1.5)b NT 
rad17Δ 30 (9)d <14 (<4)b NT 
rad24Δ 40 (11)d <26 (<7.4)b NT 
Others    
sgs1Δ 77 (22)e 60 (17) <74 (<21)b 
rfa1-t11 (CEN) 420 (120)a <69 (<20)b NT 
rad27Δ 4,400 (1,257)e NT <277 (<79)b 
tsa1Δ 173 (49)f NT <84 (24)b 
pif1Δ 3,530 (1,008)c 519 (148) 200 (57) 
Relevant genotype Mutation rate (Canr − 5FOAr) (×10−10
 CDC28 cdc28-as1 cdc28-5M 
WT 3.50 (1)a <12.5 (<3.6)b <128 (<37)b 
CKIs    
sic1Δ 59.5 (17) NT NT 
far1Δ 71.1 (20) NT NT 
sic1Δ far1Δ 115.9 (33) NT NT 
swe1Δ <5.69 (<1.6)b NT NT 
HR    
rad50Δ 2,300 (657)a 148 (42) NT 
mre11Δ 2,490 (711) 128 (37) <327 (93)b 
mre11Δ in nocodazoleg 5,700 (1,622) NT NT 
mre11Δ in SC, RTh 1,890 (543) NT NT 
mre11Δ yku80Δ 6,156 (1,759) 542 (155) NT 
rad52Δ 350 (100)c 290 (83) <446 (127)b 
sae2Δ 177 (51) <67 (<19)b NT 
sae2Δ mre11Δ 2,798 (797) NT NT 
DNA damage and replication checkpoint    
mec1Δ 680 (194)d NT <33 (<9)b 
mec1Δ tel1Δ 45,000 (12,857)d NT 2,390 (683) 
rad53Δ 95 (27)d <9.6 (<2.7)b NT 
pds1Δ 670 (190)d <64 (<18)b NT 
rfc5-1Δ 660 (189)d <5.1 (<1.5)b NT 
rad17Δ 30 (9)d <14 (<4)b NT 
rad24Δ 40 (11)d <26 (<7.4)b NT 
Others    
sgs1Δ 77 (22)e 60 (17) <74 (<21)b 
rfa1-t11 (CEN) 420 (120)a <69 (<20)b NT 
rad27Δ 4,400 (1,257)e NT <277 (<79)b 
tsa1Δ 173 (49)f NT <84 (24)b 
pif1Δ 3,530 (1,008)c 519 (148) 200 (57) 

NT, not tested. Numbers in parentheses indicate fold increase over WT. The mutation rates are shown as events per generation.

a

Data from Chen and Kolodner (1999).

b

GCR rates could not be accurately calculated because GCRs were not detected in a large enough proportion of the cultures.

c

Data from Myung et al. (2001a).

d

Data from Myung et al. (2001c).

e

Data from Myung et al. (2001b).

f

Data from Huang and Kolodner (2005).

g

mre11Δ mutants were grown in 10 µM nocodazole until stationary phase to test the effect of delaying the cell cycle on GCR rates.

h

mre11Δ mutants were grown in synthetic complete medium (SC) at RT until stationary phase to test the effect of delaying the cell cycle on GCR rates.

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