Table I. Composition of primary and... | Rockefeller University Press

Table I.

Composition of primary and secondary lymphatic organs of Bmf-deficient mice

Cell populations	WT	bmf ^−/−	bim ^−/−
Thymus
Total cellularity (×10⁷)	26.56 ± 2.28	29.07 ± 2.44	22.47 ± 3.29
CD4⁺ cells	2.38 ± 0.19	1.94 ± 0.16	4.85 ± 0.81^{^a}
CD8⁺ cells	0.93 ± 0.08	0.84 ± 0.09	1.6 ± 0.23^{^a}
CD4⁺8⁺ cells	22.28 ± 2.02	25.25 ± 2.17	13.84 ± 2.13^{^a}
CD4−8⁻ cells	0.98 ± 0.1	1.03 ± 0.11	2.15 ± 0.28^{^a}
Bone marrow
Total cellularity (×10⁶)	31.37 ± 2.4	40.19 ± 2.44^{^a}	34.7 ± 2.63
T cells	1.26 ± 0.15	1.45 ± 0.09	2.85 ± 0.31^{^a}
Myeloid cells	12.91 ± 1.41	15.12 ± 1.42	9.73 ± 0.75
Erythroid progenitors	12.13 ± 1.11	10.27 ± 0.82	6.78 ± 0.62^{^a}
B cells	8.87 ± 1.15	15.57 ± 1.28^{^a}	10.67 ± 0.93
Pro B cells	1.61 ± 0.23	1.78 ± 0.11	2.61 ± 0.37^{^a}
Pre B cells	4.66 ± 0.49	6.69 ± 0.52^{^a}	4.94 ± 0.62
T1 B cells	1.06 ± 0.19	1.86 ± 0.22^{^a}	1.32 ± 0.43
Mature B cells	1.73 ± 0.19	4.29 ± 0.48^{^a}	4.07 ± 0.99^{^a}
Spleen
Total cellularity (×10⁷)	16.19 ± 1.42	26.1 ± 2.13^{^b}	36.86 ± 3.32^{^a}
CD4⁺ T cells	2.76 ± 0.25	2.84 ± 0.24	5.12 ± 0.42^{^a}
CD8⁺ T cells	1.7 ± 0.3	2.01 ± 0.18	4.21 ± 0.41^{^a}
Myeloid cells	1.1 ± 0.21	1.3 ± 0.17	1.07 ± 0.12
Erythroid progenitors	1.67 ± 0.39	2.09 ± 0.36	3.59 ± 0.32^{^a}
B cells	9.56 ± 0.98	18.79 ± 0.15^{^b}	22.3 ± 2.13^{^b}
White blood cells
Total cellularity (×10⁶/ml)	8.76 ± 0.89	12.31 ± 0.91	22.4 ± 3.83^{^a}
CD4⁺ T cells	1.22 ± 0.26	0.96 ± 0.14	2.02 ± 0.31
CD8⁺ T cells	0.97 ± 0.2	0.84 ± 0.07	2.77 ± 0.38^{^a}
Myeloid cells	1.56 ± 0.3	1.12 ± 0.13	1.41 ± 0.22
B cells	5.71 ± 0.62	9.01 ± 0.79^{^a}	16.25 ± 2.86^{^a}
T1 B cells	0.51 ± 0.08	0.8 ± 0.1^{^a}	1.47 ± 0.31^{^a}
Inguinal lymph nodes
Total cellularity (×10⁶)	9.75 ± 1.05	17.81 ± 1.47^{^a}	21.25 ± 3.55^{^a}
CD4⁺ T cells	3.98 ± 0.51	5.44 ± 0.48	7.86 ± 1.09^{^a}
CD8⁺ T cells	2.67 ± 0.29	4.51 ± 0.42^{^a}	11.99 ± 1.41^{^a}
B cells	2.75 ± 0.58	7.7 ± 0.92^{^a}	5.47 ± 0.97^{^a}

Cell populations	WT	bmf ^−/−	bim ^−/−
Thymus
Total cellularity (×10⁷)	26.56 ± 2.28	29.07 ± 2.44	22.47 ± 3.29
CD4⁺ cells	2.38 ± 0.19	1.94 ± 0.16	4.85 ± 0.81^{^a}
CD8⁺ cells	0.93 ± 0.08	0.84 ± 0.09	1.6 ± 0.23^{^a}
CD4⁺8⁺ cells	22.28 ± 2.02	25.25 ± 2.17	13.84 ± 2.13^{^a}
CD4−8⁻ cells	0.98 ± 0.1	1.03 ± 0.11	2.15 ± 0.28^{^a}
Bone marrow
Total cellularity (×10⁶)	31.37 ± 2.4	40.19 ± 2.44^{^a}	34.7 ± 2.63
T cells	1.26 ± 0.15	1.45 ± 0.09	2.85 ± 0.31^{^a}
Myeloid cells	12.91 ± 1.41	15.12 ± 1.42	9.73 ± 0.75
Erythroid progenitors	12.13 ± 1.11	10.27 ± 0.82	6.78 ± 0.62^{^a}
B cells	8.87 ± 1.15	15.57 ± 1.28^{^a}	10.67 ± 0.93
Pro B cells	1.61 ± 0.23	1.78 ± 0.11	2.61 ± 0.37^{^a}
Pre B cells	4.66 ± 0.49	6.69 ± 0.52^{^a}	4.94 ± 0.62
T1 B cells	1.06 ± 0.19	1.86 ± 0.22^{^a}	1.32 ± 0.43
Mature B cells	1.73 ± 0.19	4.29 ± 0.48^{^a}	4.07 ± 0.99^{^a}
Spleen
Total cellularity (×10⁷)	16.19 ± 1.42	26.1 ± 2.13^{^b}	36.86 ± 3.32^{^a}
CD4⁺ T cells	2.76 ± 0.25	2.84 ± 0.24	5.12 ± 0.42^{^a}
CD8⁺ T cells	1.7 ± 0.3	2.01 ± 0.18	4.21 ± 0.41^{^a}
Myeloid cells	1.1 ± 0.21	1.3 ± 0.17	1.07 ± 0.12
Erythroid progenitors	1.67 ± 0.39	2.09 ± 0.36	3.59 ± 0.32^{^a}
B cells	9.56 ± 0.98	18.79 ± 0.15^{^b}	22.3 ± 2.13^{^b}
White blood cells
Total cellularity (×10⁶/ml)	8.76 ± 0.89	12.31 ± 0.91	22.4 ± 3.83^{^a}
CD4⁺ T cells	1.22 ± 0.26	0.96 ± 0.14	2.02 ± 0.31
CD8⁺ T cells	0.97 ± 0.2	0.84 ± 0.07	2.77 ± 0.38^{^a}
Myeloid cells	1.56 ± 0.3	1.12 ± 0.13	1.41 ± 0.22
B cells	5.71 ± 0.62	9.01 ± 0.79^{^a}	16.25 ± 2.86^{^a}
T1 B cells	0.51 ± 0.08	0.8 ± 0.1^{^a}	1.47 ± 0.31^{^a}
Inguinal lymph nodes
Total cellularity (×10⁶)	9.75 ± 1.05	17.81 ± 1.47^{^a}	21.25 ± 3.55^{^a}
CD4⁺ T cells	3.98 ± 0.51	5.44 ± 0.48	7.86 ± 1.09^{^a}
CD8⁺ T cells	2.67 ± 0.29	4.51 ± 0.42^{^a}	11.99 ± 1.41^{^a}
B cells	2.75 ± 0.58	7.7 ± 0.92^{^a}	5.47 ± 0.97^{^a}

Total numbers of hemopoietic cells from 6–8-wk-old animals were calculated by counting single-cell suspensions derived from the indicated organs and tissues that were subsequently analyzed by flow cytometry after staining for cell surface markers: total T cells (Thy-1⁺), total myeloid cells (Mac-1⁺), total nucleated erythroid progenitor cells (Ter119⁺), total B cells (B220⁺), pro–B cells (B220⁺CD43⁺IgM⁻), pre–B cells (B220⁺CD43⁻IgM⁻), T1 B cells (sIgM^highCD21^low), and mature B cells (sIgM^lowIgD⁺). Numbers represent the mean (± the SEM) of 6 WT, 9 bmf^−/−, and 5 bim^−/− mice. Total cellularity of both inguinal lymph nodes and femora are presented.

P < 0.05 compared to WT mice.

P < 0.0001 compared to WT mice.

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