Plasmids used in this study
| Plasmid | Description | Source |
| pPL2612 | vps36ΔUb (T187F G187A) | This study |
| pPL2613 | VPS36 (−309–1,698 VPS36 and 840 bp of the PHO8 3′UT) in pRS316 | This study |
| pPL2612 | vps36ΔUb (T187F/G187A) in pRS316 | This study |
| pPL2645 | vps36ΔUb (T187F/G187A) as a NotI–Acc65I fragment from pPL2612 in pRS304 | This study |
| pPL2646 | VPS36 as a NotI–Acc65I fragment from pPL2613 in pRS304 | This study |
| pPL2940 | vps23ΔUb1 in pRS306 (F52AQ117AW125A) | This study |
| pPL2180 | VPS23 (−598–1,298) as an XbaI–SacI fragment in pRS316 with BamHI site removed from polylinker with an XbaI–XhoI fill-in ligation | This study |
| pPL2447 | vps23 with UEV mutation F52A in pPL2180 | This study |
| pPL2448 | vps23 with UEV mutation N123A in pPL2180 | This study |
| pPL2449 | vps23 with UEV mutation W125A in pPL2180 | This study |
| pPL2451 | vps23 with UEV mutation S55AD56A in pPL2180 | This study |
| pPL2452 | vps23 with UEV mutation F52AS55AD56A in pPL2180 | This study |
| pPL2453 | vps23 with UEV mutation S55AD56AN123A in pPL2180 | This study |
| pPL2465 | vps23 with UEV mutation S55A in pPL2180 | This study |
| pPL2466 | vps23 with UEV mutation S55AF105A in pPL2180 | This study |
| pPL2567 | vps23 with UEV mutation F105AW125A in pPL2180 | This study |
| pPL2568 | vps23 with UEV mutation F52AF105A in pPL2180 | This study |
| pPL2569 | vps23 with UEV mutation F105A in pPL2180 | This study |
| pPL2570 | vps23 with UEV mutation G57A in pPL2180 | This study |
| pPL2571 | vps23 with UEV mutation G57AF105A in pPL2180 | This study |
| pPL2578 | vps23 with UEV mutation F52AW125A in pPL2180 | This study |
| pPL2651 | vps23 with UEV mutation F52AN123AW125A in pPL2180 | This study |
| pPL2652 | vps23ΔUb1, UEV mutation F52AQ117AW125A in pPL2180 | This study |
| pPL2653 | vps23ΔUb2, UEV mutation F52AM107AW125A in pPL2180 | This study |
| pPL2654 | vps23 with UEV mutation F52AL102AW125A in pPL2180 | This study |
| pPL2655 | vps23 with UEV mutation Q117A in pPL2180 | This study |
| pPL2940 | vps23ΔUb1 from pPL2652 in pRS306 | This study |
| pPL2941 | vps23ΔUb2 from pPL2653 in pRS306 | This study |
| pPL3355 | GST-Vps36 GLUE domain (residues 1–289) | This study |
| pPL3358 | GST-Vps36ΔUb GLUE domain (residues 1–289) | This study |
| pPL3353 | VPS36 GLUE domain (residues 1–289) in pET151 | This study |
| pPL3356 | vps36ΔUb (residues 1–289) in pET151 | This study |
| pPL2251 | vps27ΔVps23 in pRS306 PSD1 and PSD2 motifs mutated (AA448ADP and P524AAA) | This study |
| pPL3230 | MVB12 (aa 1–101) in pET151 | This study |
| pPL3203 | MVB12 from S. kluyveri (aa 1–102) in pET151 | This study |
| pPL3284 | MVB12 residues 56–101 in pET151 | This study |
| pPL3543 | MVB12 residues 56–101 F71DQWYK-A71AAAA in pET151 | This study |
| pPL3542 | MVB12 residues 56–101 L92L-D92Q in pET151 | This study |
| pPL3544 | MVB12 residues 56–101 W74Y-R74S in pET151 | This study |
| pPL3545 | MVB12 residues 56–101 K82KPPG-D82DGGT in pET151 | This study |
| pPL3551 | MVB12 residues 56–101 Y79LSKKPP-A79AAAA in pET151 | This study |
| pPL3552 | MVB12 residues 56–101 F71D-S71N in pET151 | This study |
| pPL3709 | mvb12ΔUb1 Y79LSKKPP-A79AAAA with single HA tag in pRS316 | This study |
| pPL3711 | mvb12ΔUb2 K82KPPG-D82DGGT with single HA tag in pRS316 | This study |
| pPL3713 | MVB12 with single HA tag in pRS316 | This study |
| pPL3780 | mvb12ΔUb2 K82KPPG-D82DGGT in pRS306 | This study |
| pPL3781 | mvb12ΔUb1 Y79LSKKPP-A79AAAA in pRS306 | This study |
| pPL3784 | MVB12 in pRS306 | This study |
| NA | MVB12-TAP (TAP-tagged MVB12 driven by PRC1 promoter) | Oestreich et al., 2007 |
| pAO94 | mvb12ΔUb1 Y79LSKKPP-A79AAAA -TAP (TAP-tagged mvb12 mutant driven by PRC1 promoter) | This study |
| NA | MVB12-GFP (GFP-tagged MVB12 driven by PRC1 promoter) | Oestreich et al., 2007 |
| pAO104 | mvb12ΔUb1 Y79LSKKPP-A79AAAA -GFP (GFP-tagged mvb12 mutant driven by PRC1 promoter) | This study |
| NA | Ub-GST; expression plasmid for Ub fused to GST | Katzmann et al., 2001 |
| pPL1945 | GST-Vps27; expression plasmid for residues 353–610 of Vps27 fused to GST | Bilodeau et al., 2003 |
| pGEX3x | GST expression plasmid | Smith and Johnson, 1988 |
| NA | VPS23-GFP (GFP-tagged Vps23 driven by the endogenous promoter in pRS416) | Nickerson et al., 2006 |
| pPL3982 | Vps23ΔUb1-GFP (GFP-tagged Vps23ΔUb1 driven by endogenous promoter) | This study |
| pPL3979 | Vps20-mCherry (mCherry-tagged Vps20 under the control of the endogenous promoter in pRS316) | This study |
| Plasmid | Description | Source |
| pPL2612 | vps36ΔUb (T187F G187A) | This study |
| pPL2613 | VPS36 (−309–1,698 VPS36 and 840 bp of the PHO8 3′UT) in pRS316 | This study |
| pPL2612 | vps36ΔUb (T187F/G187A) in pRS316 | This study |
| pPL2645 | vps36ΔUb (T187F/G187A) as a NotI–Acc65I fragment from pPL2612 in pRS304 | This study |
| pPL2646 | VPS36 as a NotI–Acc65I fragment from pPL2613 in pRS304 | This study |
| pPL2940 | vps23ΔUb1 in pRS306 (F52AQ117AW125A) | This study |
| pPL2180 | VPS23 (−598–1,298) as an XbaI–SacI fragment in pRS316 with BamHI site removed from polylinker with an XbaI–XhoI fill-in ligation | This study |
| pPL2447 | vps23 with UEV mutation F52A in pPL2180 | This study |
| pPL2448 | vps23 with UEV mutation N123A in pPL2180 | This study |
| pPL2449 | vps23 with UEV mutation W125A in pPL2180 | This study |
| pPL2451 | vps23 with UEV mutation S55AD56A in pPL2180 | This study |
| pPL2452 | vps23 with UEV mutation F52AS55AD56A in pPL2180 | This study |
| pPL2453 | vps23 with UEV mutation S55AD56AN123A in pPL2180 | This study |
| pPL2465 | vps23 with UEV mutation S55A in pPL2180 | This study |
| pPL2466 | vps23 with UEV mutation S55AF105A in pPL2180 | This study |
| pPL2567 | vps23 with UEV mutation F105AW125A in pPL2180 | This study |
| pPL2568 | vps23 with UEV mutation F52AF105A in pPL2180 | This study |
| pPL2569 | vps23 with UEV mutation F105A in pPL2180 | This study |
| pPL2570 | vps23 with UEV mutation G57A in pPL2180 | This study |
| pPL2571 | vps23 with UEV mutation G57AF105A in pPL2180 | This study |
| pPL2578 | vps23 with UEV mutation F52AW125A in pPL2180 | This study |
| pPL2651 | vps23 with UEV mutation F52AN123AW125A in pPL2180 | This study |
| pPL2652 | vps23ΔUb1, UEV mutation F52AQ117AW125A in pPL2180 | This study |
| pPL2653 | vps23ΔUb2, UEV mutation F52AM107AW125A in pPL2180 | This study |
| pPL2654 | vps23 with UEV mutation F52AL102AW125A in pPL2180 | This study |
| pPL2655 | vps23 with UEV mutation Q117A in pPL2180 | This study |
| pPL2940 | vps23ΔUb1 from pPL2652 in pRS306 | This study |
| pPL2941 | vps23ΔUb2 from pPL2653 in pRS306 | This study |
| pPL3355 | GST-Vps36 GLUE domain (residues 1–289) | This study |
| pPL3358 | GST-Vps36ΔUb GLUE domain (residues 1–289) | This study |
| pPL3353 | VPS36 GLUE domain (residues 1–289) in pET151 | This study |
| pPL3356 | vps36ΔUb (residues 1–289) in pET151 | This study |
| pPL2251 | vps27ΔVps23 in pRS306 PSD1 and PSD2 motifs mutated (AA448ADP and P524AAA) | This study |
| pPL3230 | MVB12 (aa 1–101) in pET151 | This study |
| pPL3203 | MVB12 from S. kluyveri (aa 1–102) in pET151 | This study |
| pPL3284 | MVB12 residues 56–101 in pET151 | This study |
| pPL3543 | MVB12 residues 56–101 F71DQWYK-A71AAAA in pET151 | This study |
| pPL3542 | MVB12 residues 56–101 L92L-D92Q in pET151 | This study |
| pPL3544 | MVB12 residues 56–101 W74Y-R74S in pET151 | This study |
| pPL3545 | MVB12 residues 56–101 K82KPPG-D82DGGT in pET151 | This study |
| pPL3551 | MVB12 residues 56–101 Y79LSKKPP-A79AAAA in pET151 | This study |
| pPL3552 | MVB12 residues 56–101 F71D-S71N in pET151 | This study |
| pPL3709 | mvb12ΔUb1 Y79LSKKPP-A79AAAA with single HA tag in pRS316 | This study |
| pPL3711 | mvb12ΔUb2 K82KPPG-D82DGGT with single HA tag in pRS316 | This study |
| pPL3713 | MVB12 with single HA tag in pRS316 | This study |
| pPL3780 | mvb12ΔUb2 K82KPPG-D82DGGT in pRS306 | This study |
| pPL3781 | mvb12ΔUb1 Y79LSKKPP-A79AAAA in pRS306 | This study |
| pPL3784 | MVB12 in pRS306 | This study |
| NA | MVB12-TAP (TAP-tagged MVB12 driven by PRC1 promoter) | Oestreich et al., 2007 |
| pAO94 | mvb12ΔUb1 Y79LSKKPP-A79AAAA -TAP (TAP-tagged mvb12 mutant driven by PRC1 promoter) | This study |
| NA | MVB12-GFP (GFP-tagged MVB12 driven by PRC1 promoter) | Oestreich et al., 2007 |
| pAO104 | mvb12ΔUb1 Y79LSKKPP-A79AAAA -GFP (GFP-tagged mvb12 mutant driven by PRC1 promoter) | This study |
| NA | Ub-GST; expression plasmid for Ub fused to GST | Katzmann et al., 2001 |
| pPL1945 | GST-Vps27; expression plasmid for residues 353–610 of Vps27 fused to GST | Bilodeau et al., 2003 |
| pGEX3x | GST expression plasmid | Smith and Johnson, 1988 |
| NA | VPS23-GFP (GFP-tagged Vps23 driven by the endogenous promoter in pRS416) | Nickerson et al., 2006 |
| pPL3982 | Vps23ΔUb1-GFP (GFP-tagged Vps23ΔUb1 driven by endogenous promoter) | This study |
| pPL3979 | Vps20-mCherry (mCherry-tagged Vps20 under the control of the endogenous promoter in pRS316) | This study |
NA, not applicable.