Table I.

Lethality of different ECTV isolates

Experiment
Virus
Dose (PFU)
Dead/total
1 Rev166 0.1 2/6 
  1 3/6 
  300 5/5 
 WT 0.1 2/6 
  1 4/4 
  10 4/4 
  300 5/5 
 Δ166 300 0/5 
  106 0/5 
  107 0/4 
  5 × 107 0/3 
2 Rev166 0.1 1/6 
  1 2/6 
 WT 1 6/6 
 Δ166 5 × 107 0/4 
3 Rev166 0.2 2/6 
  2 6/6 
 WT 0.2 5/6 
 Δ166 5 × 107 0/4 
 189898-p7.5-EGFP 0.5 2/6 
  5 6/6 

Mice were infected with the indicated viruses serially diluted in PBS from stocks. For experiments 1 and 2, the dose was calculated according to the virus titer determined in the virus stock. For experiment 3, final dilutions were retitered. Based on the results, we estimate LD50 values for the different viruses as follows: Rev166, <2; WT, <0.2; p7.5-EGFP, <5; Δ166, >5 × 107. These experiments are independent of those in Fig. 2 C.

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