Table I. Antiinflammatory activity of... | Rockefeller University Press

Table I.

Antiinflammatory activity of chemerin-derived peptides

Percentage inhibition of LPS/IFN-γ–induced inflammatory cytokine expression
Cytokine	Chemerin	C6	C8	C11	C13	C15	C15-S	C15-M	C19	N19	M20
TNF-α	70	0	0	0	10	61	0	0	21	0	0
RANTES	40	0	0	0	32	47	0	0	41	0	0
IL-1β	60	—	—	—	—	54	—	—	—	—	—
IL-12p40	54	—	—	—	—	47	—	—	—	—	—
IL-6	42	—	—	—	—	43	—	—	—	—	—

Percentage inhibition of LPS/IFN-γ–induced inflammatory cytokine expression
Cytokine	Chemerin	C6	C8	C11	C13	C15	C15-S	C15-M	C19	N19	M20
TNF-α	70	0	0	0	10	61	0	0	21	0	0
RANTES	40	0	0	0	32	47	0	0	41	0	0
IL-1β	60	—	—	—	—	54	—	—	—	—	—
IL-12p40	54	—	—	—	—	47	—	—	—	—	—
IL-6	42	—	—	—	—	43	—	—	—	—	—

PMΦs were challenged with 100 ng/ml LPS and 20 ng/ml IFN-γ for 15 h with or without pretreatment with peptides (0.1 pM–100 nM) for 1 h. Where peptides exhibited antiinflammatory properties, percentage inhibition of LPS/IFN-γ–induced MΦ activation represents effect with optimal dose (1 pM chemerin and C15 or 100 pM C13 and C19). Peptide sequences are as follows: C6 (R¹⁵⁷-K¹⁶²; RALRTK), C8 (F¹⁵⁵-K¹⁶²; FSRALRTK), C11 (P¹⁴⁴-A¹⁵⁴; PHGYFLPGQFA), C13 (P¹⁴⁴-S¹⁵⁶; PHGYFLPGQFAFS), C15 (A¹⁴⁰-A¹⁵⁴; AGEDPHGYFLPGQFA), C19 (A¹³⁸-S¹⁵⁶; AQAGEDPHGYFLPGQFAFS), N19 (E²³-K⁴¹; ELSETQRRSLQVALEEFHK), and M20 (K⁸⁶-K¹⁰⁵; KPECTIKPNGRRRKCLACIK). Control peptides: C15-S (GLFHDQAGPPAGYEF) and C15-M (AGEDPHGYALPGQAA; F148A and F153A). Data represent mean percentage inhibition of cytokine production by classically activated MΦs from four to eight independent experiments as determined by ELISAs and Luminex assays.

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