Table II. Summary of spectratype... | Rockefeller University Press

Table II.

Summary of spectratype analysis of purified PA-specific T cells^{^a}

Mouse number	T cell receptor Vβ^{^b}
	1	2	3.1	4	5.1	5.2	5.3	6	7	8.1	8.2	8.3	9	10	11	12	13	14	15	16	18	20
Young
Y1	-	-	-	1^{^c}	-	1	-	2	1	-	-	4	-	-	1	2	-	-	-	-	-	-
Y2	-	-	1	-	-	2	-	1	3	-	-	-	-	1	2	-	-	-	1	1	-	1
Y3	-	-	-	1	-	1	-	2	3	-	-	-	1	-	1	1	1	-	-	1	-	-
Y4	1	-	-	1	-	1	-	1	4	2	-	-	2	1	1	1	-	-	1	2	-	-
Aged
A1	1	1	-	1	-	4	-	1	4	1	-	-	1	2	1	1	-	1	2	1	1	1
A2	1	2	-	1	-	1	-	1	2	2	1	4	4	1	1	2	-	1	2	1	-	-
A3	-	-	-	-	-	-	-	1	1	-	1	3	1	-	1	-	-	-	1	-	-	-
A4	-	1	-	-	-	2	-	2	3	-	1	3	1	1	1	-	1	-	-	1	1	-
A5	2	1	1	3	-	-	-	1	2	1	-	-	1	1	1	2	-	1	1	2	-	-
A6	-	-	-	2	-	-	-	3	3	-	-	-	2	-	-	1	-	-	-	2	-	-

Mouse number	T cell receptor Vβ^{^b}
	1	2	3.1	4	5.1	5.2	5.3	6	7	8.1	8.2	8.3	9	10	11	12	13	14	15	16	18	20
Young
Y1	-	-	-	1^{^c}	-	1	-	2	1	-	-	4	-	-	1	2	-	-	-	-	-	-
Y2	-	-	1	-	-	2	-	1	3	-	-	-	-	1	2	-	-	-	1	1	-	1
Y3	-	-	-	1	-	1	-	2	3	-	-	-	1	-	1	1	1	-	-	1	-	-
Y4	1	-	-	1	-	1	-	1	4	2	-	-	2	1	1	1	-	-	1	2	-	-
Aged
A1	1	1	-	1	-	4	-	1	4	1	-	-	1	2	1	1	-	1	2	1	1	1
A2	1	2	-	1	-	1	-	1	2	2	1	4	4	1	1	2	-	1	2	1	-	-
A3	-	-	-	-	-	-	-	1	1	-	1	3	1	-	1	-	-	-	1	-	-	-
A4	-	1	-	-	-	2	-	2	3	-	1	3	1	1	1	-	1	-	-	1	1	-
A5	2	1	1	3	-	-	-	1	2	1	-	-	1	1	1	2	-	1	1	2	-	-
A6	-	-	-	2	-	-	-	3	3	-	-	-	2	-	-	1	-	-	-	2	-	-

PA tetramer^⁺ CD8 T cells were sorted from individual young (2–3 mo) and aged (18 mo) mice 10 d after influenza virus infection. The percentage of PA^⁺ cells for young mice ranged between 8 and 30%, and for individual aged mice numbers 1–6 the percentages were 9.8, 19.4, 2.8. 4.4, 6.4, and 6.3%, respectively.

Spectratype analysis was carried out with a panel of 5′ Vβ and 3′ Cβ primers.

Numbers of expanded peaks (>10% of the maximal peak height) for each Vβ-Cβ.

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