Summary of spectratype analysis of purified NP-specific T cellsa
| Mouse number . | T cell receptor Vβb . | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | 1 . | 2 . | 3.1 . | 4 . | 5.1 . | 5.2 . | 5.3 . | 6 . | 7 . | 8.1 . | 8.2 . | 8.3 . | 9 . | 10 . | 11 . | 12 . | 13 . | 14 . | 15 . | 16 . | 18 . | 20 . |
| Young | ||||||||||||||||||||||
| Y1 | 1c | 1 | 2 | 1 | - | - | - | 2 | 3 | - | 1 | 1 | 1 | 1 | 1 | 2 | - | 1 | 1 | - | - | - |
| Y2 | 1 | - | - | 1 | - | - | - | 1 | 2 | - | 2 | 2 | - | 1 | - | - | - | - | 1 | - | - | - |
| Y3 | 1 | 1 | 2 | 1 | - | 1 | - | 1 | 1 | - | 1 | 1 | - | 3 | - | 1 | - | - | 2 | 3 | - | - |
| Y4 | 2 | 1 | 4 | 2 | - | 2 | - | - | 2 | 2 | 1 | 1 | 3 | - | - | 4 | - | - | 2 | 3 | - | - |
| Y5 | 3 | 1 | 1 | 2 | - | 1 | - | 2 | 2 | 1 | 2 | 2 | - | 3 | 1 | 1 | - | - | 2 | 2 | - | - |
| Aged | ||||||||||||||||||||||
| A1 | 1 | - | - | 1 | - | - | - | 1 | - | - | 1 | 1 | - | 1 | - | - | - | - | 1 | 1 | 1 | 1 |
| A2 | 1 | - | - | 1 | - | 1 | - | 1 | - | - | 1 | 1 | 1 | 1 | 2 | 2 | - | 1 | 1 | 1 | - | - |
| A3 | - | - | - | - | - | 1 | - | 1 | 1 | - | - | - | - | - | - | 1 | - | - | - | - | - | - |
| A4 | - | - | - | 2 | - | 1 | - | - | 1 | - | 1 | - | - | - | - | - | - | - | - | - | - | - |
| A5 | 3 | 1 | - | 1 | - | 1 | - | 2 | 2 | 1 | 1 | 2 | 2 | 2 | 2 | - | - | 2 | - | 1 | - | - |
| A6 | - | - | - | - | - | 1 | - | - | 1 | - | - | 1 | - | - | - | 2 | - | - | - | - | - | - |
| Mouse number . | T cell receptor Vβb . | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | 1 . | 2 . | 3.1 . | 4 . | 5.1 . | 5.2 . | 5.3 . | 6 . | 7 . | 8.1 . | 8.2 . | 8.3 . | 9 . | 10 . | 11 . | 12 . | 13 . | 14 . | 15 . | 16 . | 18 . | 20 . |
| Young | ||||||||||||||||||||||
| Y1 | 1c | 1 | 2 | 1 | - | - | - | 2 | 3 | - | 1 | 1 | 1 | 1 | 1 | 2 | - | 1 | 1 | - | - | - |
| Y2 | 1 | - | - | 1 | - | - | - | 1 | 2 | - | 2 | 2 | - | 1 | - | - | - | - | 1 | - | - | - |
| Y3 | 1 | 1 | 2 | 1 | - | 1 | - | 1 | 1 | - | 1 | 1 | - | 3 | - | 1 | - | - | 2 | 3 | - | - |
| Y4 | 2 | 1 | 4 | 2 | - | 2 | - | - | 2 | 2 | 1 | 1 | 3 | - | - | 4 | - | - | 2 | 3 | - | - |
| Y5 | 3 | 1 | 1 | 2 | - | 1 | - | 2 | 2 | 1 | 2 | 2 | - | 3 | 1 | 1 | - | - | 2 | 2 | - | - |
| Aged | ||||||||||||||||||||||
| A1 | 1 | - | - | 1 | - | - | - | 1 | - | - | 1 | 1 | - | 1 | - | - | - | - | 1 | 1 | 1 | 1 |
| A2 | 1 | - | - | 1 | - | 1 | - | 1 | - | - | 1 | 1 | 1 | 1 | 2 | 2 | - | 1 | 1 | 1 | - | - |
| A3 | - | - | - | - | - | 1 | - | 1 | 1 | - | - | - | - | - | - | 1 | - | - | - | - | - | - |
| A4 | - | - | - | 2 | - | 1 | - | - | 1 | - | 1 | - | - | - | - | - | - | - | - | - | - | - |
| A5 | 3 | 1 | - | 1 | - | 1 | - | 2 | 2 | 1 | 1 | 2 | 2 | 2 | 2 | - | - | 2 | - | 1 | - | - |
| A6 | - | - | - | - | - | 1 | - | - | 1 | - | - | 1 | - | - | - | 2 | - | - | - | - | - | - |
NP tetramer+ T cells were sorted from individual young (2–3 mo) and aged (18 mo) mice 10 d after influenza virus infection. The percentage of NP+ cells for young mice ranged between 15 and 30%, and for individual aged mice numbers 1–6 the percentages were 5.6, 6.3, 8.2. 1.1, 8.1, and 8.2%, respectively.
Spectratype analysis was carried out with a panel of 5′ Vβ and 3′ Cβ primers.
Numbers of expanded peaks (>10% of the maximal peak height) for each Vβ-Cβ.