Table II.

Plasmids used in this study

Plasmid Markers Source 
pRS413 CEN HIS3 Sikorski and Hieter, 1989  
pSB231 CEN URA3 fus1-LacZ Trueheart et al., 1987  
pJAU01 URA3 cdc28-as 1 Bishop et al., 2000  
pSY1 2µ LEU2 STE12 Dolan et al., 1989  
pPP2330 URA3 STE5-8A Strickfaden et al., 2007  
YCpIF5 CEN URA3 PGAL1 Foreman and Davis, 1994  
pPROTet.E133 Ptet-6xHN Cmr Clontech Laboratories, Inc. 
pMR4937 CEN URA3 FLAG-FUS3 Matheos et al., 2004  
pMR4938 CEN URA3 FLAG-fus3Q93G Matheos et al., 2004  
pMR5048 CEN HIS3 FLAG-FUS3 D. Matheosc 
pMR5469a CEN URA3 PGAL1-FUS2∷GFP104 Paterson et al., 2008  
pMR5482 CEN URA3 FUS2∷GFP104 Paterson et al., 2008  
pMR5598 pFa6a-mCherry-kanMX6 S. Clarkc 
pMR5630 Ptet-6xHN-fus21–328 Cmr This study 
pMR5642 CEN LEU2 FUS2∷GFP104 This study 
pMR5774b CEN URA3 PGAL1-fus21–104-GFP This study 
Plasmid Markers Source 
pRS413 CEN HIS3 Sikorski and Hieter, 1989  
pSB231 CEN URA3 fus1-LacZ Trueheart et al., 1987  
pJAU01 URA3 cdc28-as 1 Bishop et al., 2000  
pSY1 2µ LEU2 STE12 Dolan et al., 1989  
pPP2330 URA3 STE5-8A Strickfaden et al., 2007  
YCpIF5 CEN URA3 PGAL1 Foreman and Davis, 1994  
pPROTet.E133 Ptet-6xHN Cmr Clontech Laboratories, Inc. 
pMR4937 CEN URA3 FLAG-FUS3 Matheos et al., 2004  
pMR4938 CEN URA3 FLAG-fus3Q93G Matheos et al., 2004  
pMR5048 CEN HIS3 FLAG-FUS3 D. Matheosc 
pMR5469a CEN URA3 PGAL1-FUS2∷GFP104 Paterson et al., 2008  
pMR5482 CEN URA3 FUS2∷GFP104 Paterson et al., 2008  
pMR5598 pFa6a-mCherry-kanMX6 S. Clarkc 
pMR5630 Ptet-6xHN-fus21–328 Cmr This study 
pMR5642 CEN LEU2 FUS2∷GFP104 This study 
pMR5774b CEN URA3 PGAL1-fus21–104-GFP This study 
a

Point mutations in FUS2∷GFP used in Fig. 5 D were created for this study and are derived from pMR5469.

b

Point mutations in fus21–104-GFP used in Fig. 5 (B and C) were created for this study and are derived from pMR5774.

c

Princeton University, Princeton, NJ.

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