Summary of results for all proteins analyzed in this study
| Notes | Lipid bound | Localization | Ratio FPM/FCyt | D | τ | Range (63%) | Range (95%) | n | |
| µm2/s | s | µm | µm | ||||||
| GFP | None | None | Cytoplasm | ND | 31.36 ± 5.06 | NA | NA | NA | 13 |
| PM-YFP | None | Integral | PM | ND | 0.79 ± 0.06 | 2.12 × 1021 ± 2.11 × 1021 | ∞ | ∞ | 43 |
| GFP-PH123-MyoX | None | PIP2 + PIP3 | PM | 1.15 ± 0.04 | 0.07 ± 0.02 | 6.96 ± 0.93 | 0.98 | 1.70 | 14 |
| PH-PLCδ1-GFP | None | PIP2 | PM | 2.07 ± 0.12 | 1.24 ± 0.14 | 2.44 ± 0.27 | 2.46 | 4.26 | 52 |
| PH-PLCδ1R40L-GFP | None | None | Cytoplasm | ND | 21.47 ± 3.01 | NA | NA | NA | 21 |
| GFP-PH-PH-PLCδ1 | None | PIP2 | PM | 3.49 ± 0.17 | 0.24 ± 0.02 | 11.13 ± 1.83 | 2.29 | 3.97 | 33 |
| PH-PH-PLCδ1-GFP | None | PIP2 | PM | 4.45 ± 0.40 | 0.28 ± 0.03 | 11.08 ± 2.01 | 2.50 | 4.33 | 30 |
| PHR40L-PH-PLCδ1-GFP | None | PIP2 | PM | 2.13 ± 0.12 | 0.34 ± 0.05 | 2.87 ± 0.26 | 1.40 | 2.43 | 30 |
| PH-PLCδ1-GFP | CHO-M1 | PIP2 | PM | 1.66 ± 0.11 | 0.60 ± 0.10 | 2.21 ± 0.22 | 1.63 | 2.82 | 15 |
| GFP-PH-PH-PLCδ1 | CHO-M1 | PIP2 | PM | 2.99 ± 0.27 | 0.05 ± 0.01 | 7.03 ± 0.77 | 0.85 | 1.48 | 13 |
| GFP-GAP1m | LY | None | Cytoplasm | ND | 3.96 ± 0.66 | NA | NA | NA | 28 |
| Insulin | PIP3 | PM | 1.52 ± 0.08 | 1.33 ± 0.29 | 3.42 ± 0.44 | 3.02 | 5.23 | 30 | |
| GFP-GAP1IP4BP | LY | PIP2 | PM | 2.55 ± 0.11 | 0.35 ± 0.04 | 3.50 ± 0.26 | 1.57 | 2.72 | 40 |
| Insulin | PIP2 + PIP3 | PM | 4.44 ± 0.27 | 0.27 ± 0.02 | 6.46 ± 1.12 | 1.86 | 3.22 | 46 | |
| GFP-GAP1IP4BP | 10 µM Wmn | None | Cytoplasm | ND | 4.62 ± 0.54 | NA | NA | NA | 19 |
| GFP-GAP1IP4BP-ΔC2 | LY | PIP2 | PM | 2.33 ± 0.13 | 0.41 ± 0.04 | 3.34 ± 0.50 | 1.65 | 2.85 | 36 |
| Insulin | PIP2 + PIP3 | PM | 4.28 ± 0.27 | 0.49 ± 0.04 | 7.93 ± 1.78 | 2.79 | 4.84 | 38 | |
| GFP-PH-GAP1IP4BP | LY | PIP2 | PM | 3.31 ± 0.18 | 0.59 ± 0.04 | 3.64 ± 0.38 | 2.07 | 3.58 | 51 |
| Insulin | PIP2 + PIP3 | PM | 4.99 ± 0.37 | 0.70 ± 0.04 | 7.37 ± 1.06 | 3.21 | 5.56 | 47 |
| Notes | Lipid bound | Localization | Ratio FPM/FCyt | D | τ | Range (63%) | Range (95%) | n | |
| µm2/s | s | µm | µm | ||||||
| GFP | None | None | Cytoplasm | ND | 31.36 ± 5.06 | NA | NA | NA | 13 |
| PM-YFP | None | Integral | PM | ND | 0.79 ± 0.06 | 2.12 × 1021 ± 2.11 × 1021 | ∞ | ∞ | 43 |
| GFP-PH123-MyoX | None | PIP2 + PIP3 | PM | 1.15 ± 0.04 | 0.07 ± 0.02 | 6.96 ± 0.93 | 0.98 | 1.70 | 14 |
| PH-PLCδ1-GFP | None | PIP2 | PM | 2.07 ± 0.12 | 1.24 ± 0.14 | 2.44 ± 0.27 | 2.46 | 4.26 | 52 |
| PH-PLCδ1R40L-GFP | None | None | Cytoplasm | ND | 21.47 ± 3.01 | NA | NA | NA | 21 |
| GFP-PH-PH-PLCδ1 | None | PIP2 | PM | 3.49 ± 0.17 | 0.24 ± 0.02 | 11.13 ± 1.83 | 2.29 | 3.97 | 33 |
| PH-PH-PLCδ1-GFP | None | PIP2 | PM | 4.45 ± 0.40 | 0.28 ± 0.03 | 11.08 ± 2.01 | 2.50 | 4.33 | 30 |
| PHR40L-PH-PLCδ1-GFP | None | PIP2 | PM | 2.13 ± 0.12 | 0.34 ± 0.05 | 2.87 ± 0.26 | 1.40 | 2.43 | 30 |
| PH-PLCδ1-GFP | CHO-M1 | PIP2 | PM | 1.66 ± 0.11 | 0.60 ± 0.10 | 2.21 ± 0.22 | 1.63 | 2.82 | 15 |
| GFP-PH-PH-PLCδ1 | CHO-M1 | PIP2 | PM | 2.99 ± 0.27 | 0.05 ± 0.01 | 7.03 ± 0.77 | 0.85 | 1.48 | 13 |
| GFP-GAP1m | LY | None | Cytoplasm | ND | 3.96 ± 0.66 | NA | NA | NA | 28 |
| Insulin | PIP3 | PM | 1.52 ± 0.08 | 1.33 ± 0.29 | 3.42 ± 0.44 | 3.02 | 5.23 | 30 | |
| GFP-GAP1IP4BP | LY | PIP2 | PM | 2.55 ± 0.11 | 0.35 ± 0.04 | 3.50 ± 0.26 | 1.57 | 2.72 | 40 |
| Insulin | PIP2 + PIP3 | PM | 4.44 ± 0.27 | 0.27 ± 0.02 | 6.46 ± 1.12 | 1.86 | 3.22 | 46 | |
| GFP-GAP1IP4BP | 10 µM Wmn | None | Cytoplasm | ND | 4.62 ± 0.54 | NA | NA | NA | 19 |
| GFP-GAP1IP4BP-ΔC2 | LY | PIP2 | PM | 2.33 ± 0.13 | 0.41 ± 0.04 | 3.34 ± 0.50 | 1.65 | 2.85 | 36 |
| Insulin | PIP2 + PIP3 | PM | 4.28 ± 0.27 | 0.49 ± 0.04 | 7.93 ± 1.78 | 2.79 | 4.84 | 38 | |
| GFP-PH-GAP1IP4BP | LY | PIP2 | PM | 3.31 ± 0.18 | 0.59 ± 0.04 | 3.64 ± 0.38 | 2.07 | 3.58 | 51 |
| Insulin | PIP2 + PIP3 | PM | 4.99 ± 0.37 | 0.70 ± 0.04 | 7.37 ± 1.06 | 3.21 | 5.56 | 47 |
All experiments were in HEK cells unless otherwise stated. The localization of the protein (either plasma membrane [PM] or cytoplasm) is noted, along with the lipid bound (PtdIns(4,5)P2 or PtdIns(3,4,5)P3). The parameters are the ratio of fluorescence at the PM verses the cytoplasm (ratio of FPM/FCyt) as a crude index of membrane recruitment, lateral diffusion coefficient (D), and membrane dissociation time constant (τ). The range refers to the expected distance traveled on the membrane for the proportion of these proteins indicated in parentheses, and are estimated as √(2 × D × τ) for 63% and √(2 × D × 3 × τ) for 95%, respectively (Teruel and Meyer, 2000). NA, not applicable; ND, not determined.