Kinetic events of NE formation
| . | Nuclear accumulation . | . | . | . | Rim formation . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | From chromosome segregation . | Standard deviation . | n . | P-value . | From chromosome segregation . | Standard deviation . | n . | P-value . | ||||||
| Wild type | 10.2 min | 0.2 min | 85 | n/a | 11.9 min | 2.3 min | 28 | n/a | ||||||
| V5-Rtn3 | 21.9 min | 2.0 min | 49 | <0.001 | nd | |||||||||
| V5-Rtn4 | 26.3 min | 2.4 min | 51 | <0.001 | 25.4 min | 13.8 min | 29 | <0.001 | ||||||
| V5-DP1 | 25.2 min | 3.8 min | 34 | <0.001 | nd | |||||||||
| Scramble RNA | 10.6 min | 0.4 min | 19 | 0.38 | 12.9 min | 2.7 min | 20 | 0.2 | ||||||
| Reticulon siRNA | 7.8 min | 0.2 min | 57 | <0.001 | 9.7 min | 2.2 min | 17 | 0.003 | ||||||
| . | Nuclear accumulation . | . | . | . | Rim formation . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | From chromosome segregation . | Standard deviation . | n . | P-value . | From chromosome segregation . | Standard deviation . | n . | P-value . | ||||||
| Wild type | 10.2 min | 0.2 min | 85 | n/a | 11.9 min | 2.3 min | 28 | n/a | ||||||
| V5-Rtn3 | 21.9 min | 2.0 min | 49 | <0.001 | nd | |||||||||
| V5-Rtn4 | 26.3 min | 2.4 min | 51 | <0.001 | 25.4 min | 13.8 min | 29 | <0.001 | ||||||
| V5-DP1 | 25.2 min | 3.8 min | 34 | <0.001 | nd | |||||||||
| Scramble RNA | 10.6 min | 0.4 min | 19 | 0.38 | 12.9 min | 2.7 min | 20 | 0.2 | ||||||
| Reticulon siRNA | 7.8 min | 0.2 min | 57 | <0.001 | 9.7 min | 2.2 min | 17 | 0.003 | ||||||
Time from chromosome segregation to nuclear accumulation and NE rim formation are represented as mean values in minutes. Standard deviation is provided alone with number of cells measured (n) and t test–calculated p-values. n/a, not applicable; nd, not determined.