Table II. The effect of serine point... | Rockefeller University Press

Table II.

The effect of serine point mutations on the interaction between MBD-ank and the ankyrin-binding motif of sodium channels

GST-Na_v 1.2 1,080–1,203 mutants	k_on	k_off	K_D
	M⁻¹s⁻¹	s⁻¹	M
S1112A	8.9 ± 0.2 ×10⁵	3.3 ± 0.4 × 10⁻³	3.7 ± 0.3 × 10⁻⁹
S1123A	19.1 × 10⁵	3.4 × 10⁻³	1.8 × 10⁻⁹^{^a}
S1124A	16.3 ± 3.2 × 10⁵	3.9 ± 0.3 × 10⁻³	2.5 ± 0.7 × 10⁻⁹
S1126A	16.2 ± 14.4 × 10⁵	3.1 ± 0.8 × 10⁻³	2.6 ± 1.3 × 10⁻⁹
S1123-24A	8.2 ± 2.6 × 10⁵	3.4 ± 0.2 × 10⁻³	4.5 ± 1.3 × 10⁻⁹
S1124-26A	0.9 ± 1 × 10⁵	4.2 ± 1.6 × 10⁻³	1.6 ± 1.3 × 10⁻⁷
S1112-24-26A	NA	NA	2.6 ± 1.1 × 10⁻⁶^{^b}

GST-Na_v 1.2 1,080–1,203 mutants	k_on	k_off	K_D
	M⁻¹s⁻¹	s⁻¹	M
S1112A	8.9 ± 0.2 ×10⁵	3.3 ± 0.4 × 10⁻³	3.7 ± 0.3 × 10⁻⁹
S1123A	19.1 × 10⁵	3.4 × 10⁻³	1.8 × 10⁻⁹^{^a}
S1124A	16.3 ± 3.2 × 10⁵	3.9 ± 0.3 × 10⁻³	2.5 ± 0.7 × 10⁻⁹
S1126A	16.2 ± 14.4 × 10⁵	3.1 ± 0.8 × 10⁻³	2.6 ± 1.3 × 10⁻⁹
S1123-24A	8.2 ± 2.6 × 10⁵	3.4 ± 0.2 × 10⁻³	4.5 ± 1.3 × 10⁻⁹
S1124-26A	0.9 ± 1 × 10⁵	4.2 ± 1.6 × 10⁻³	1.6 ± 1.3 × 10⁻⁷
S1112-24-26A	NA	NA	2.6 ± 1.1 × 10⁻⁶^{^b}

Mean values ± SD are obtained from three to five experiments. NA, not applicable.

Value from one experiment.

K_D values were calculated using steady-state analysis (see Materials and methods).