ADC of single molecules and percentage of the different diffusion modes
| Proteins and treatments . | D ± SD . | DBrownian ± SD . | Brownian . | Confined . | Mixed . |
|---|---|---|---|---|---|
| μm2/s | μm2/s | μm2/s | % | % | |
| CD9 | 0.23 ± 0.15 | 0.33 ± 0.12 | 38 (5) | 23 (18) | 39 (30) |
| CD9plm | 0.28 ± 0.18a | 0.43 ± 0.18b | 38 (4) | 21 (14) | 41 (22) |
| CD9 + MβCD | 0.08 ± 0.09b | 0.19 ± 0.12b | 32 (3) | 31 (19) | 37 (18) |
| CD9 + MβCD–Chl | 0.41 ± 0.33b | 0.64 ± 0.23b | 40 (8) | 28 (23) | 32 (28) |
| CD55 | 0.24 ± 0.26 | 0.37 ± 0.31 | 40 (4) | 18 (4) | 42 (9) |
| CD55 + MβCD | 0.06 ± 0.05b | 0.08 ± 0.06 | 47 (ND) | 19 (ND) | 34 (ND) |
| CD46 | 0.13 ± 0.08b | 0.18 ± 0.07 | 52 (ND) | 13 (ND) | 35 (ND) |
| CD46 + MβCD | 0.05 ± 0.05b | 0.09 ± 0.04 | 39 (ND) | 27 (ND) | 34 (ND) |
| Proteins and treatments . | D ± SD . | DBrownian ± SD . | Brownian . | Confined . | Mixed . |
|---|---|---|---|---|---|
| μm2/s | μm2/s | μm2/s | % | % | |
| CD9 | 0.23 ± 0.15 | 0.33 ± 0.12 | 38 (5) | 23 (18) | 39 (30) |
| CD9plm | 0.28 ± 0.18a | 0.43 ± 0.18b | 38 (4) | 21 (14) | 41 (22) |
| CD9 + MβCD | 0.08 ± 0.09b | 0.19 ± 0.12b | 32 (3) | 31 (19) | 37 (18) |
| CD9 + MβCD–Chl | 0.41 ± 0.33b | 0.64 ± 0.23b | 40 (8) | 28 (23) | 32 (28) |
| CD55 | 0.24 ± 0.26 | 0.37 ± 0.31 | 40 (4) | 18 (4) | 42 (9) |
| CD55 + MβCD | 0.06 ± 0.05b | 0.08 ± 0.06 | 47 (ND) | 19 (ND) | 34 (ND) |
| CD46 | 0.13 ± 0.08b | 0.18 ± 0.07 | 52 (ND) | 13 (ND) | 35 (ND) |
| CD46 + MβCD | 0.05 ± 0.05b | 0.09 ± 0.04 | 39 (ND) | 27 (ND) | 34 (ND) |
D is the ADC mean value of all trajectories (corresponding to the three modes of diffusion), and DBrownian is the ADC mean value of pure Brownian trajectories. Numbers in parentheses correspond to the percentage of total trajectories associated with TEAs. Error estimates represent the SD for the different cells analyzed.
P < 10−3; a different distribution as compared with control cells (CD9) using the Mann-Whitney U test.
P < 10−4; a different distribution as compared with control cells (CD9) using the Mann-Whitney U test.