Gradual overexpression of UbcH10 leads to progressive aneuploidy
| Mitotic MEF genotype | Aneuploid figures (SD) | Karyotypes with indicated chromosome number | Mitotic figures with PMSCS (SD) | |||||||||||
| 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 48 | 52 | |||
| % | % | |||||||||||||
| +/+ | 13 (1) | 1 | 4 | 6 | 130 | 6 | 3 | 2 (1) | ||||||
| UbcH10T1 | 28 (2) | 5 | 2 | 9 | 108 | 9 | 6 | 6 | 2 | 1 | 2 | 3 (0) | ||
| UbcH10T2 | 29 (2) | 2 | 4 | 10 | 107 | 6 | 9 | 3 | 4 | 1 | 1 | 1 | 2 | 6 (2) |
| UbcH10T1/T1 | 31 (2) | 3 | 4 | 9 | 103 | 16 | 5 | 7 | 3 | 5 (2) | ||||
| UbcH10T2/T2 | 33 (4) | 3 | 13 | 100 | 13 | 7 | 8 | 3 | 2 | 1 | 5 (2) | |||
| Mitotic MEF genotype | Aneuploid figures (SD) | Karyotypes with indicated chromosome number | Mitotic figures with PMSCS (SD) | |||||||||||
| 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 48 | 52 | |||
| % | % | |||||||||||||
| +/+ | 13 (1) | 1 | 4 | 6 | 130 | 6 | 3 | 2 (1) | ||||||
| UbcH10T1 | 28 (2) | 5 | 2 | 9 | 108 | 9 | 6 | 6 | 2 | 1 | 2 | 3 (0) | ||
| UbcH10T2 | 29 (2) | 2 | 4 | 10 | 107 | 6 | 9 | 3 | 4 | 1 | 1 | 1 | 2 | 6 (2) |
| UbcH10T1/T1 | 31 (2) | 3 | 4 | 9 | 103 | 16 | 5 | 7 | 3 | 5 (2) | ||||
| UbcH10T2/T2 | 33 (4) | 3 | 13 | 100 | 13 | 7 | 8 | 3 | 2 | 1 | 5 (2) | |||
Of each genotype, 50 spreads from three individual cell lines were counted. Aneuploidy and PMSCS were measured at passage 5. Empty cells indicate that there were no karyotypes with the indicated chromosome number.