Cargo secretion accelerated upon ADF and cofilin1 knockdown (class C)
| Protein name | Unique peptides | Ratio (H/L) control/RNAi |
| Thrombospondin 3 | 3 | 0.3 |
| Follistatin-related protein 4 | 10 | 0.3 |
| Thrombospondin 1 | 12 | 0.4 |
| Pappalysin-1 | 4 | 0.4 |
| Serine protease HTRA1 | 6 | 0.4 |
| Laminin subunit β1 | 20 | 0.5 |
| Laminin subunit α4 | 16 | 0.5 |
| Proprotein convertase subtilisin/kexin type 9 | 8 | 0.5 |
| Metalloproteinase inhibitor 1 (TIMP1) | 2 | 0.5 |
| Nidogen-1 | 6 | 0.5 |
| SPARC-related modular calcium-binding protein 1 | 3 | 0.4 |
| Laminin subunit γ1 | 18 | 0.6 |
| Laminin subunit β1 | 20 | 0.5 |
| Galectin-3–binding protein | 3 | 0.6 |
| 72 kD type IV collagenase | 2 | 0.6 |
| Stanniocalcin-2 | 1 | 0.6 |
| Cystatin-C | 1 | 0.6 |
| Cab45 (Golgi) | 2 | 0.6 |
| α-Galactosidase A (lysosome) | 2 | 0.6 |
| Protein name | Unique peptides | Ratio (H/L) control/RNAi |
| Thrombospondin 3 | 3 | 0.3 |
| Follistatin-related protein 4 | 10 | 0.3 |
| Thrombospondin 1 | 12 | 0.4 |
| Pappalysin-1 | 4 | 0.4 |
| Serine protease HTRA1 | 6 | 0.4 |
| Laminin subunit β1 | 20 | 0.5 |
| Laminin subunit α4 | 16 | 0.5 |
| Proprotein convertase subtilisin/kexin type 9 | 8 | 0.5 |
| Metalloproteinase inhibitor 1 (TIMP1) | 2 | 0.5 |
| Nidogen-1 | 6 | 0.5 |
| SPARC-related modular calcium-binding protein 1 | 3 | 0.4 |
| Laminin subunit γ1 | 18 | 0.6 |
| Laminin subunit β1 | 20 | 0.5 |
| Galectin-3–binding protein | 3 | 0.6 |
| 72 kD type IV collagenase | 2 | 0.6 |
| Stanniocalcin-2 | 1 | 0.6 |
| Cystatin-C | 1 | 0.6 |
| Cab45 (Golgi) | 2 | 0.6 |
| α-Galactosidase A (lysosome) | 2 | 0.6 |
Secretion of proteins into cell culture media from control and ADF and cofilin1 siRNA–treated HeLa cells was analyzed by SILAC-based MS. There was an accelerated secretion of cargoes classified as class C upon ADF and cofilin1 knockdown (ratio between heavy and light peptides [H/L] < 1). Cab45, a luminal Golgi-resident protein, and α-galactosidase A (lysosomal hydrolase) were secreted upon ADF and cofilin1 knockdown.