Table II.

Cargoes inhibited in secretion upon ADF and cofilin1 knockdown (class B)

Protein name Unique peptides Ratio (H/L) control/RNAi 
Secretogranin-2 11 
Collagen α1 (III) chain 4.6 
Lumican 
Collagen α1 (I) chain 2.8 
Collagen α1(XVIII) chain 
Agrin (secreted form) 16 1.9 
Collagen α1(VII) chain 42 1.9 
Collagen α1(XV) chain 1.9 
Immunoglobulin superfamily member 10 1.8 
Perlecan 51 1.6 
Protein name Unique peptides Ratio (H/L) control/RNAi 
Secretogranin-2 11 
Collagen α1 (III) chain 4.6 
Lumican 
Collagen α1 (I) chain 2.8 
Collagen α1(XVIII) chain 
Agrin (secreted form) 16 1.9 
Collagen α1(VII) chain 42 1.9 
Collagen α1(XV) chain 1.9 
Immunoglobulin superfamily member 10 1.8 
Perlecan 51 1.6 

Secretion of proteins into cell culture media from control and ADF and cofilin1 siRNA–treated HeLa cells was analyzed by SILAC-based MS. There was an inhibition of secretion of cargoes classified as class B upon ADF and cofilin1 knockdown (ratio between heavy and light peptides [H/L] > 1).

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