Quantification of the mitotic defects in control or ISWI RNAi S2 cells at 4 da
| Prophase | Prometaphase | Metaphase | Anaphase | Telophase | Mono/multipolar spindles | |
| Control RNAi (GFP) | 7.9 ± 2.9 | 9.9 ± 0.2 | 23.3 ± 0.4 | 9.9 ± 1.3 | 41.1 ± 1.3 | 6.4 ± 3.4 |
| ISWI RNAi (MRC015_H12) | 6.3 ± 0.4 | 11.2 ± 1.1 | 23.2 ± 4.5 | 3.4 ± 0.5 | 13.6 ± 1.9 | 7.3 ± 0.4 |
| 1b | 2b | 3b | 4b | Mitotic index | ||
| Control RNAi (GFP) | 1.0 ± 0.1 | 0 | 0 | 0 | 3.3 ± 0.1 | |
| ISWI RNAi (MRC015_H12) | 6.7 ± 1.8 | 17.9 ± 5.6 | 2.9 ± 0.1 | 7.4 ± 6.5 | 3.0 ± 0.9 |
| Prophase | Prometaphase | Metaphase | Anaphase | Telophase | Mono/multipolar spindles | |
| Control RNAi (GFP) | 7.9 ± 2.9 | 9.9 ± 0.2 | 23.3 ± 0.4 | 9.9 ± 1.3 | 41.1 ± 1.3 | 6.4 ± 3.4 |
| ISWI RNAi (MRC015_H12) | 6.3 ± 0.4 | 11.2 ± 1.1 | 23.2 ± 4.5 | 3.4 ± 0.5 | 13.6 ± 1.9 | 7.3 ± 0.4 |
| 1b | 2b | 3b | 4b | Mitotic index | ||
| Control RNAi (GFP) | 1.0 ± 0.1 | 0 | 0 | 0 | 3.3 ± 0.1 | |
| ISWI RNAi (MRC015_H12) | 6.7 ± 1.8 | 17.9 ± 5.6 | 2.9 ± 0.1 | 7.4 ± 6.5 | 3.0 ± 0.9 |
Values represent the percentage of each mitotic phase or classified phenotype over the total number of mitotic cells. The cells at 4 d after dsRNA treatment were immunostained and counted under a microscope (more than 100 mitotic cells per sample). Errors represent SD from two independent experiments.
Abnormal mitotic cells were classified into four phenotypes (Fig. 10 E).