Table I.

Strains and plasmids used in this study

Strain/plasmid Genotype or description Source 
SEY6210 MATa ura3-52, his3-Δ200, leu2-3, -112 trp1-Δ901, suc2-Δ9, lys2-801 C. Beha 
CBY926 SEY6210 osh1Δ::kan-MX4 osh2 Δ::kan-MX4 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3 / osh4-1, CEN-TRP1 C. Beh 
SEY6218 MATa ura3-52, his3-Δ200, leu2-3, -112 trp1-Δ901, suc2-Δ9, lys2-801 S. Emrb 
AAY102 SEY6218 stt4::HIS3 / pRS415 CEN-LEU2-stt4-4 S. Emr 
SD102 MATa leu2 ura3 rme1 trp1 his3Δ GAL+ TOF3 mss4::HIS3MX6 / YCplac111::mss4-2ts C. Jacksonc 
CVY215 Mat a leu2-3 -112 his 3-11-15 trp1-1 ura3-1 ade2-1 /ss-RFP-HDEL:TRP1 This study 
WPY979 CVY215 HXT3-GFP:HIS3 This study 
WPY1009 CVY215 RTN1-GFP:HIS3 This study 
CVY433 CVY215 cho1::kan-MX4 This study 
NDY93 MATa sec14-3 osh4::kan-MX4 ura3-1 his 3-11, -15 leu2-5, 112 Laboratory collection 
NDY75 CBY926 upc2-1:URA3 Laboratory collection 
pJK59 Sec63-GFP, SEC63 promoter (URA3/CEN) Laboratory collection 
pCV19 Rtn1-GFP, RTN1 promoter (URA3/CEN) Laboratory collection 
pTL312 PHO5 promoter, GFP- Osh2p (URA3/CEN) T. Levined 
pTL313 PHO5 promoter, GFP-Osh3p (URA3/CEN) T. Levine 
YIplac204/TKC-DsRed-HDEL Encodes ss-RFP-HDEL (TRP) B. Glicke 
Strain/plasmid Genotype or description Source 
SEY6210 MATa ura3-52, his3-Δ200, leu2-3, -112 trp1-Δ901, suc2-Δ9, lys2-801 C. Beha 
CBY926 SEY6210 osh1Δ::kan-MX4 osh2 Δ::kan-MX4 osh3Δ::LYS2 osh4Δ::HIS3 osh5Δ::LEU2 osh6Δ::LEU2 osh7Δ::HIS3 / osh4-1, CEN-TRP1 C. Beh 
SEY6218 MATa ura3-52, his3-Δ200, leu2-3, -112 trp1-Δ901, suc2-Δ9, lys2-801 S. Emrb 
AAY102 SEY6218 stt4::HIS3 / pRS415 CEN-LEU2-stt4-4 S. Emr 
SD102 MATa leu2 ura3 rme1 trp1 his3Δ GAL+ TOF3 mss4::HIS3MX6 / YCplac111::mss4-2ts C. Jacksonc 
CVY215 Mat a leu2-3 -112 his 3-11-15 trp1-1 ura3-1 ade2-1 /ss-RFP-HDEL:TRP1 This study 
WPY979 CVY215 HXT3-GFP:HIS3 This study 
WPY1009 CVY215 RTN1-GFP:HIS3 This study 
CVY433 CVY215 cho1::kan-MX4 This study 
NDY93 MATa sec14-3 osh4::kan-MX4 ura3-1 his 3-11, -15 leu2-5, 112 Laboratory collection 
NDY75 CBY926 upc2-1:URA3 Laboratory collection 
pJK59 Sec63-GFP, SEC63 promoter (URA3/CEN) Laboratory collection 
pCV19 Rtn1-GFP, RTN1 promoter (URA3/CEN) Laboratory collection 
pTL312 PHO5 promoter, GFP- Osh2p (URA3/CEN) T. Levined 
pTL313 PHO5 promoter, GFP-Osh3p (URA3/CEN) T. Levine 
YIplac204/TKC-DsRed-HDEL Encodes ss-RFP-HDEL (TRP) B. Glicke 
a

Simon Frasier University, Vancouver, British Columbia, Canada.

b

Cornell University, Ithaca, NY.

c

Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.

d

Institute of Ophthalmology, University College London, London, England, UK.

e

University of Chicago, Chicago, IL.

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