Table I.

List of strains used

Strain name Mating type Genotype 
CRLg48 h+ his2-245-245 leu1-32 lys1+::LEU2 
CRLh20 h− leu1-32 lys1+::LEU2 lys3 ura1 
CRLi50 h90 leu1-32 ura4-D18 ade6-210 sad1-mRFP::kan lys1+::taz1-GFP taz1Δ::ura4+ 
CRLk22 h90 leu1-32 ura4-D18 ade6-216 sad1-mRFP::kan lys1+::taz1-GFP taz1Δ::ura4+ bqt2Δ::ura4+ 
CRLl02 h+ his2-245 leu1-32 
CRLl12 h+ his2-245 leu1-32 bqt3Δ::LEU2 
CRLl58 h+ his2-245 leu1-32 bqt3Δ::LEU2 lys3 ura1 
CRLl88 h+ his2-245 leu1-32 lys1-131 ade6-216 
CRLm03 h90 ura4-D18 bqt2Δ::ura4+ lys1+ 
CRLp44 h− ura4-D18 bqt4Δ::ura4+ 
CRLp67 h+ his2-245 leu1-32 lys1-131 ura4-D18 ade6-216 bqt4Δ::ura4+ 
CRLp83 h90 leu1-32 ura4-D18 ade6-216 lys1+::taz1-mRFP bqt2-GFP::kan taz1Δ::ura4+ bqt4Δ::ura4+ 
CRLp85 h90 leu1-32 ura4-D18 ade6-216 sad1-mRFP::kan lys1+::taz1-GFP taz1Δ::ura4+ bqt4Δ::ura4+ 
CRLp86 h90 ura4-D18 bqt4Δ::ura4+ 
CRLp93 h− leu1-32 ura4-D18 bqt4Δ::ura4+ bqt3Δ::LEU2 lys1+::GFP-bqt4 
CRLr06 h− ura4-D18 bqt4Δ::ura4+ lys1+::GFP-bqt4 
CRLr24 h− ura4-D18 bqt4Δ::ura4+ lys1+::GFP–bqt4-dTM 
CRLr25 h− leu1-32 ura4-D18 bqt4Δ::ura4+ lys1+::GFP–bqt4-dTM bqt3Δ::LEU2 
CRLr75 h90 leu1-32 lys1-131 ura4-D18 ade6-210 hus5+::ccr1N-GFP::LEU2 taz1-mCherry::kan 
CRLr81 h90 leu1-32 lys1-131 ura4-D18 ade6-210 bqt4Δ::ura4+ hus5+::ccr1N-GFP::LEU2 taz1-mCherry::kan 
CRLr82 h90 leu1-32 lys1-131 ura4-D18 ade6-216 bqt2Δ::ura4+ hus5+::ccr1N-GFP::LEU2 taz1-mCherry::kan 
CRLs25 h90 leu1-32 ura4-D18 ade6-210 bqt4Δ::ura4+ lys1+::GFP–bqt4-dN taz1-mCherry::kan 
CRLs27 h−/h− leu1-32/leu1-32 ura4-D18/ura4-D18 ade6-210/ade6-216 sad1-mRFP::kan/sad1-mRFP::kan lys1+::CFP-bqt1/lys1-131 rap1-GFP::LEU2/rap1+ 
CRLs28 h−/h− leu1-32/leu1-32 ura4-D18/ura4-D18 ade6-210/ade6-216 sad1-mRFP::kan/sad1-mRFP::kan lys1+::CFP-bqt1/lys1+::bqt2-myc rap1-GFP::LEU2/rap1+ 
CRLs29 h−/h− leu1-32/leu1-32 ura4-D18/ura4-D18 ade6-210/ade6-216 sad1-mRFP::kan/sad1-mRFP::kan lys1+::CFP-bqt1/lys1-131 rap1-GFP::LEU2/rap1+ bqt4D::ura4/bqt4D::ura4+ 
CRLs30 h−/h− leu1-32/leu1-32 ura4-D18/ura4-D18 ade6-210/ade6-216 sad1-mRFP::kan/sad1-mRFP::kan lys1+::CFP-bqt1/lys1+::bqt2-myc rap1-GFP::LEU2/rap1+ bqt4Δ::ura4/bqt4Δ::ura4+ 
CRLs33 h90 leu1-32 ura4-D18 ade6-210 bqt4Δ::ura4+ taz1-mCherry::kan lys1+::rap1-GFP–bqt4-dN 
CRLs38 h90 leu1-32 ura4-D18 ade6-210 bqt4Δ::ura4+ taz1-mCherry::kan lys1+::GFP–bqt4-dTM 
CRLs53 h90 leu1-32 ura4-D18 ade6-210 bqt4Δ::ura4+ taz1-mCherry::kan lys1+::GFP-bqt4 
CRLs57 h90 ura4-D18 bqt3Δ::ura4+ 
CRLs63 h90 leu1-32 ura4-D18 ade6-210 sad1-mRFP::kan lys1+::taz1-GFP taz1Δ::ura4+ bqt3Δ::ura4+ 
CRLs87 h− leu1-32 ura4-D18 bqt3Δ::LEU2 aur1::GFP-bqt3 bqt4Δ::ura4+ 
CRLs95 h90 ura4-D18 lys1+::ura4+ 
CRLt01 h− leu1-32 lys1-131 ura4-D18 bqt3Δ::LEU2 aur1::GFP-bqt3 lys1::ura4+ 
CRLt07 h+ his2-245 leu1-32 ura4-D18 bqt4Δ::ura4+ 
CRLt39 h− leu1-32 bqt3Δ::LEU2 
CRLt53 h− leu1-32 ura4-D18 bqt3Δ::LEU2 bqt4Δ::ura4+ aur1r::mCherry-bqt3 lys1+::GFP-bqt4 
CRLt54 h− leu1-32 ura4-D18 bqt3Δ::LEU2 bqt4Δ::ura4+ aur1r::mCherry-bqt3 lys1+::GFP–bqt4-dTM 
CRLt55 h− leu1-32 ura4-D18 bqt3Δ::LEU2 bqt4Δ::ura4+ aur1r::mCherry-bqt3 lys1+::GFP–bqt4-dN 
CRLt61 h− leu1-32 lys1-131 ura4-D18 ade6-210 bqt4Δ::ura4+ nup211-40 
CRLt62 h− leu1-32 lys1-131 ura4-D18 ade6-210 nup211-40 
CRLt63 h− leu1-32 lys1-131 ura4-D18 ade6-210 bqt3Δ::LEU2 nup211-40 
CRLt73 h90 leu1-32 lys1-131 ura4-D18 ade6-216 taz1-mCherry::kan aur1r::GFP-bqt3 
CRLu61 h− leu1-32 ura4-D18 bqt4Δ::ura4+ bqt3Δ::LEU2 lys1+::GFP-bqt4 aur1r::mCherry-bqt3 
CRLu62 h− leu1-32 ura4-D18 bqt4Δ::ura4+ bqt3Δ::LEU2 lys1+::GFP–bqt4-dN aur1r::mCherry-bqt3 
CRLu63 h− leu1-32 ura4-D18 bqt4Δ::ura4+ bqt3Δ::LEU2 lys1+::GFP–bqt4-dTM aur1r::mCherry-bqt3 
CRLu69 h− leu1-32 lys1-131 ura4-D18 bqt4Δ::ura4+ bqt3Δ::LEU2 aur1r::mCherry-bqt3 
CRLw70 h− leu1-32 ura4-swi6Δ::kan TAS-ura4+-tel2(L) 
CRLw71 h− leu1-32 ura4-TAS-ura4+-tel2(L) 
CRLw73 h− leu1-32 ura4-taz1Δ::kan TAS-ura4+-tel2(L) 
CRLw95 h− leu1-32 ura4-bqt4Δ::hyg TAS-ura4+-tel2(L) 
CRLx01 h90 leu1-32 lys1-131 ura4-D18 ade6-210 bqt4Δ::ura4+ rap1Δ::ura4+ taz1-mCherry::kan lys1::rap1-GFP–bqt4-dN 
CRLx02 h90 leu1-32 lys1-131 ura4-D18 ade6-210 bqt4Δ::ura4+ rap1Δ::ura4+ taz1-mCherry::kan lys1::rap1-GFP 
CRLx03 h90 leu1-32 lys1-131 ura4-D18 ade6-210 rap1Δ::ura4+ taz1-mCherry::kan lys1::rap1-GFP–bqt4-dN 
CRLx04 h90 leu1-32 lys1-131 ura4-D18 ade6-210 rap1Δ::ura4+ taz1-mCherry::kan lys1::rap1-GFP–bqt4-dN 
Strain name Mating type Genotype 
CRLg48 h+ his2-245-245 leu1-32 lys1+::LEU2 
CRLh20 h− leu1-32 lys1+::LEU2 lys3 ura1 
CRLi50 h90 leu1-32 ura4-D18 ade6-210 sad1-mRFP::kan lys1+::taz1-GFP taz1Δ::ura4+ 
CRLk22 h90 leu1-32 ura4-D18 ade6-216 sad1-mRFP::kan lys1+::taz1-GFP taz1Δ::ura4+ bqt2Δ::ura4+ 
CRLl02 h+ his2-245 leu1-32 
CRLl12 h+ his2-245 leu1-32 bqt3Δ::LEU2 
CRLl58 h+ his2-245 leu1-32 bqt3Δ::LEU2 lys3 ura1 
CRLl88 h+ his2-245 leu1-32 lys1-131 ade6-216 
CRLm03 h90 ura4-D18 bqt2Δ::ura4+ lys1+ 
CRLp44 h− ura4-D18 bqt4Δ::ura4+ 
CRLp67 h+ his2-245 leu1-32 lys1-131 ura4-D18 ade6-216 bqt4Δ::ura4+ 
CRLp83 h90 leu1-32 ura4-D18 ade6-216 lys1+::taz1-mRFP bqt2-GFP::kan taz1Δ::ura4+ bqt4Δ::ura4+ 
CRLp85 h90 leu1-32 ura4-D18 ade6-216 sad1-mRFP::kan lys1+::taz1-GFP taz1Δ::ura4+ bqt4Δ::ura4+ 
CRLp86 h90 ura4-D18 bqt4Δ::ura4+ 
CRLp93 h− leu1-32 ura4-D18 bqt4Δ::ura4+ bqt3Δ::LEU2 lys1+::GFP-bqt4 
CRLr06 h− ura4-D18 bqt4Δ::ura4+ lys1+::GFP-bqt4 
CRLr24 h− ura4-D18 bqt4Δ::ura4+ lys1+::GFP–bqt4-dTM 
CRLr25 h− leu1-32 ura4-D18 bqt4Δ::ura4+ lys1+::GFP–bqt4-dTM bqt3Δ::LEU2 
CRLr75 h90 leu1-32 lys1-131 ura4-D18 ade6-210 hus5+::ccr1N-GFP::LEU2 taz1-mCherry::kan 
CRLr81 h90 leu1-32 lys1-131 ura4-D18 ade6-210 bqt4Δ::ura4+ hus5+::ccr1N-GFP::LEU2 taz1-mCherry::kan 
CRLr82 h90 leu1-32 lys1-131 ura4-D18 ade6-216 bqt2Δ::ura4+ hus5+::ccr1N-GFP::LEU2 taz1-mCherry::kan 
CRLs25 h90 leu1-32 ura4-D18 ade6-210 bqt4Δ::ura4+ lys1+::GFP–bqt4-dN taz1-mCherry::kan 
CRLs27 h−/h− leu1-32/leu1-32 ura4-D18/ura4-D18 ade6-210/ade6-216 sad1-mRFP::kan/sad1-mRFP::kan lys1+::CFP-bqt1/lys1-131 rap1-GFP::LEU2/rap1+ 
CRLs28 h−/h− leu1-32/leu1-32 ura4-D18/ura4-D18 ade6-210/ade6-216 sad1-mRFP::kan/sad1-mRFP::kan lys1+::CFP-bqt1/lys1+::bqt2-myc rap1-GFP::LEU2/rap1+ 
CRLs29 h−/h− leu1-32/leu1-32 ura4-D18/ura4-D18 ade6-210/ade6-216 sad1-mRFP::kan/sad1-mRFP::kan lys1+::CFP-bqt1/lys1-131 rap1-GFP::LEU2/rap1+ bqt4D::ura4/bqt4D::ura4+ 
CRLs30 h−/h− leu1-32/leu1-32 ura4-D18/ura4-D18 ade6-210/ade6-216 sad1-mRFP::kan/sad1-mRFP::kan lys1+::CFP-bqt1/lys1+::bqt2-myc rap1-GFP::LEU2/rap1+ bqt4Δ::ura4/bqt4Δ::ura4+ 
CRLs33 h90 leu1-32 ura4-D18 ade6-210 bqt4Δ::ura4+ taz1-mCherry::kan lys1+::rap1-GFP–bqt4-dN 
CRLs38 h90 leu1-32 ura4-D18 ade6-210 bqt4Δ::ura4+ taz1-mCherry::kan lys1+::GFP–bqt4-dTM 
CRLs53 h90 leu1-32 ura4-D18 ade6-210 bqt4Δ::ura4+ taz1-mCherry::kan lys1+::GFP-bqt4 
CRLs57 h90 ura4-D18 bqt3Δ::ura4+ 
CRLs63 h90 leu1-32 ura4-D18 ade6-210 sad1-mRFP::kan lys1+::taz1-GFP taz1Δ::ura4+ bqt3Δ::ura4+ 
CRLs87 h− leu1-32 ura4-D18 bqt3Δ::LEU2 aur1::GFP-bqt3 bqt4Δ::ura4+ 
CRLs95 h90 ura4-D18 lys1+::ura4+ 
CRLt01 h− leu1-32 lys1-131 ura4-D18 bqt3Δ::LEU2 aur1::GFP-bqt3 lys1::ura4+ 
CRLt07 h+ his2-245 leu1-32 ura4-D18 bqt4Δ::ura4+ 
CRLt39 h− leu1-32 bqt3Δ::LEU2 
CRLt53 h− leu1-32 ura4-D18 bqt3Δ::LEU2 bqt4Δ::ura4+ aur1r::mCherry-bqt3 lys1+::GFP-bqt4 
CRLt54 h− leu1-32 ura4-D18 bqt3Δ::LEU2 bqt4Δ::ura4+ aur1r::mCherry-bqt3 lys1+::GFP–bqt4-dTM 
CRLt55 h− leu1-32 ura4-D18 bqt3Δ::LEU2 bqt4Δ::ura4+ aur1r::mCherry-bqt3 lys1+::GFP–bqt4-dN 
CRLt61 h− leu1-32 lys1-131 ura4-D18 ade6-210 bqt4Δ::ura4+ nup211-40 
CRLt62 h− leu1-32 lys1-131 ura4-D18 ade6-210 nup211-40 
CRLt63 h− leu1-32 lys1-131 ura4-D18 ade6-210 bqt3Δ::LEU2 nup211-40 
CRLt73 h90 leu1-32 lys1-131 ura4-D18 ade6-216 taz1-mCherry::kan aur1r::GFP-bqt3 
CRLu61 h− leu1-32 ura4-D18 bqt4Δ::ura4+ bqt3Δ::LEU2 lys1+::GFP-bqt4 aur1r::mCherry-bqt3 
CRLu62 h− leu1-32 ura4-D18 bqt4Δ::ura4+ bqt3Δ::LEU2 lys1+::GFP–bqt4-dN aur1r::mCherry-bqt3 
CRLu63 h− leu1-32 ura4-D18 bqt4Δ::ura4+ bqt3Δ::LEU2 lys1+::GFP–bqt4-dTM aur1r::mCherry-bqt3 
CRLu69 h− leu1-32 lys1-131 ura4-D18 bqt4Δ::ura4+ bqt3Δ::LEU2 aur1r::mCherry-bqt3 
CRLw70 h− leu1-32 ura4-swi6Δ::kan TAS-ura4+-tel2(L) 
CRLw71 h− leu1-32 ura4-TAS-ura4+-tel2(L) 
CRLw73 h− leu1-32 ura4-taz1Δ::kan TAS-ura4+-tel2(L) 
CRLw95 h− leu1-32 ura4-bqt4Δ::hyg TAS-ura4+-tel2(L) 
CRLx01 h90 leu1-32 lys1-131 ura4-D18 ade6-210 bqt4Δ::ura4+ rap1Δ::ura4+ taz1-mCherry::kan lys1::rap1-GFP–bqt4-dN 
CRLx02 h90 leu1-32 lys1-131 ura4-D18 ade6-210 bqt4Δ::ura4+ rap1Δ::ura4+ taz1-mCherry::kan lys1::rap1-GFP 
CRLx03 h90 leu1-32 lys1-131 ura4-D18 ade6-210 rap1Δ::ura4+ taz1-mCherry::kan lys1::rap1-GFP–bqt4-dN 
CRLx04 h90 leu1-32 lys1-131 ura4-D18 ade6-210 rap1Δ::ura4+ taz1-mCherry::kan lys1::rap1-GFP–bqt4-dN 
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