Motility defects caused by mutant LC1 proteins
| Strain/mutation | Swimming velocitya | Beat frequencyb | Distance traveled/beatc | No. independent transformants analyzed | Axonemal ATPase activity |
| μm/s | Hz | μm | nmol phosphate released/min/µg protein | ||
| WT (cc124) | 129.9 ± 15.1 | 50–60 | 2.60 | NA | 0.18 |
| cwd arg7-8 (cc3395) | 115.1 ± 34.1 | ∼45 | 2.56 | NA | ND |
| Myc tag | 114.3 ± 15.8 | 45–50 | 2.54 | 9 | 0.17 |
| M182A | 81.8 ± 19.3 | 40–45 | 2.04 | 15 | 0.15 |
| M182G | 69.2 ± 18.1 | 40–45 | 1.73 | 13 | 0.18 |
| M182P | 55.7 ± 15.7 | 42–45 | 1.32 | 11 | 0.17 |
| M182*d | ND | ND | ND | 0 | ND |
| D185G | 31.5 ± 12.2 | 40–45 | 0.79 | 5 | ND |
| D185P | 38.2 ± 9.8 | 32–35 | 1.19 | 6 | ND |
| D185*d | ND | ND | ND | 0 | ND |
| R189Ae | 64.5 ± 20.9 | 35–40 | 1.84 | 26 | 0.19 |
| R189E | 32.0 ± 15.1 | 35–40 | 0.91 | 14 | 0.17 |
| R196A | 45.6 ± 13.4 | 35–40 | 1.30 | 19 | 0.17 |
| R196D | 46.7 ± 10.2 | 30–40 | 1.55 | 7 | ND |
| Strain/mutation | Swimming velocitya | Beat frequencyb | Distance traveled/beatc | No. independent transformants analyzed | Axonemal ATPase activity |
| μm/s | Hz | μm | nmol phosphate released/min/µg protein | ||
| WT (cc124) | 129.9 ± 15.1 | 50–60 | 2.60 | NA | 0.18 |
| cwd arg7-8 (cc3395) | 115.1 ± 34.1 | ∼45 | 2.56 | NA | ND |
| Myc tag | 114.3 ± 15.8 | 45–50 | 2.54 | 9 | 0.17 |
| M182A | 81.8 ± 19.3 | 40–45 | 2.04 | 15 | 0.15 |
| M182G | 69.2 ± 18.1 | 40–45 | 1.73 | 13 | 0.18 |
| M182P | 55.7 ± 15.7 | 42–45 | 1.32 | 11 | 0.17 |
| M182*d | ND | ND | ND | 0 | ND |
| D185G | 31.5 ± 12.2 | 40–45 | 0.79 | 5 | ND |
| D185P | 38.2 ± 9.8 | 32–35 | 1.19 | 6 | ND |
| D185*d | ND | ND | ND | 0 | ND |
| R189Ae | 64.5 ± 20.9 | 35–40 | 1.84 | 26 | 0.19 |
| R189E | 32.0 ± 15.1 | 35–40 | 0.91 | 14 | 0.17 |
| R196A | 45.6 ± 13.4 | 35–40 | 1.30 | 19 | 0.17 |
| R196D | 46.7 ± 10.2 | 30–40 | 1.55 | 7 | ND |
WT, wild type; NA, not applicable. Asterisks indicate stop codons.
Determined from the tracks of at least 10 cells. Tracks were plotted in Metamorph, and statistical data were calculated from xy coordinates using Cell Motility Suite. Values are given as mean ± SD.
Beat frequency was determined using the population-based fast Fourier transform method of Kamiya (2000). Each transformant was analyzed at least three times, and the range of peak values is reported.
Determined from the mean swimming velocity and lower limit of the beat frequency data.
We have not detected any transformants that incorporate LC1 truncated at either M182 or D185 into the flagellum.
Several R189A transformants exhibited a markedly lower swimming velocity of 38.0 ± 28.8 µm/s and also had highly convoluted cell tracks.