Quantification of protein accumulation during AZ assembly using NMJ in vivo imaging
| Coexpressed proteins (A × B) | Both A and B | A before B | B before A |
| BRP × DGluRIIA | BRP+/IIA+ 17/39 (44%) | BRP+/IIA− 0/39 (0%) | BRP−/IIA+ 22/39 (56%) |
| DLiprin-α × DGluRIIA | DLiprin-α+/IIA+ 16/39 (41%) | DLiprin-α+/IIA− 23/39 (59%) | DLiprin-α−/IIA+ 0/39 (0%) |
| BRP × DLiprin-α | BRP+/DLiprin-α+ 8/31 (26%) | BRP+/DLiprin-α− 0/31 (0%) | BRP−/DLiprin-α+ 23/31 (74%) |
| DGluRIIA × Cac | IIA+/Cac+ 36/62 (58%) | IIA+/Cac− 21/62 (34%) | IIA−/Cac+ 5/62 (8%) |
| BRP × Cac | BRP+/Cac+ 42/62 (68%) | BRP+/Cac− 7/62 (11%) | BRP−/Cac+ 13/62 (21%) |
| Coexpressed proteins (A × B) | Both A and B | A before B | B before A |
| BRP × DGluRIIA | BRP+/IIA+ 17/39 (44%) | BRP+/IIA− 0/39 (0%) | BRP−/IIA+ 22/39 (56%) |
| DLiprin-α × DGluRIIA | DLiprin-α+/IIA+ 16/39 (41%) | DLiprin-α+/IIA− 23/39 (59%) | DLiprin-α−/IIA+ 0/39 (0%) |
| BRP × DLiprin-α | BRP+/DLiprin-α+ 8/31 (26%) | BRP+/DLiprin-α− 0/31 (0%) | BRP−/DLiprin-α+ 23/31 (74%) |
| DGluRIIA × Cac | IIA+/Cac+ 36/62 (58%) | IIA+/Cac− 21/62 (34%) | IIA−/Cac+ 5/62 (8%) |
| BRP × Cac | BRP+/Cac+ 42/62 (68%) | BRP+/Cac− 7/62 (11%) | BRP−/Cac+ 13/62 (21%) |
Quantification of the relative accumulation of the indicated synaptic proteins at newly forming AZs (Δt = ∼12 h). A synaptic site was scored positive (+) or negative (−) for a specific protein depending on whether protein fluorescence signals exceeded the mean background level by >2.5-fold. For example, when comparing BRP and DGluRIIA (at synapses forming newly over 12 h), 44% were positive for both proteins, 0% for BRP only, and 56% for DGluRIIA only.