Table II.

Frequencies of p21-positive and/or BrdU-negative cells within the APB-positive populations after a 4-d induction of p53 and a 24-h pulse of BrdU

Cell lines (treatmentaTotal APB+ countedb p21+ (%) BrdU− (%) p21+ and BrdU− (%) 
C7 (4OHT) 150 132 (88.0) 129 (86.0) 122 (81.3) 
C8 (4OHT) 127 105 (82.7) 106 (83.5) 94 (74.0) 
IIICF-T/B3(SV40T-siRNA) 153 128 (83.7) 131 (85.6) 115 (75.2) 
Cell lines (treatmentaTotal APB+ countedb p21+ (%) BrdU− (%) p21+ and BrdU− (%) 
C7 (4OHT) 150 132 (88.0) 129 (86.0) 122 (81.3) 
C8 (4OHT) 127 105 (82.7) 106 (83.5) 94 (74.0) 
IIICF-T/B3(SV40T-siRNA) 153 128 (83.7) 131 (85.6) 115 (75.2) 
a

Cells were treated with 1 µM 4OHT or 10 nM SV40T siRNA for 4 d, and BrdU was added 24 h before the end of treatment.

b

Only cells that were APB positive were examined for BrdU and p21.

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