Table I.

Curve fit parameters for selected Qc and Sec17 dose-response relationships

Protein Figure Experimental condition EC50 IC50 Hill coefficient r2 
   nM nM   
Qcwt 2 A No ATP (priming bypass) 3.74 ± 0.66 NA 1.04 ± 0.15 0.979 
Qc7Δ 2 A No ATP (priming bypass) 0.55 ± 0.07 NA 1.03 ± 0.11 0.982 
Qc5Δ 2 A No ATP (priming bypass) 1.05 ± 0.15 NA 0.96 ± 0.11 0.982 
Qc3Δ 2 A No ATP (priming bypass) 4.75 ± 0.53 NA 1.34 ± 0.16 0.989 
Qc5Δ 2 C +ATP (competition with wt) NA 28.9 ± 7.8 −1.10 ± 0.25 0.947 
Qc3Δ 2 C +ATP (competition with wt) NA 40.6 ± 3.4 −1.30 ± 0.12 0.996 
Qc1Δ 2 C and S1 +ATP (competition with wt) NA 656 ± 110 −0.70 ± 0.08 0.733 
QcΔSD 2 C and S1 +ATP (competition with wt) NA 417 ± 45 −0.99 ± 0.10 0.841 
Qc GST-PX S1 +ATP (competition with wt) NA 2,010 ± 247 −0.89 ± 0.09 0.892 
Qcwt 6 A No ATP, +94 nM Sec17 34.3 ± 4.8 NA 0.94 ± 0.08 0.997 
Qc7Δ 6 A No ATP, +94 nM Sec17 8.6 ± 1.1 NA 1.13 ± 0.16 0.990 
Qc5Δ 6 A No ATP, +94 nM Sec17 11.7 ± 1.2 NA 1.33 ± 0.13 0.996 
Qc3Δ 6 A No ATP, +94 nM Sec17 19.9 ± 1.6 NA 1.29 ± 0.07 0.999 
Sec17 wt 6 C No ATP, +75 nM Qc3Δ 125 ± 3.0 NA 4.97 ± 0.46 0.995 
Sec17-LALA 6 C No ATP, +75 nM Qc3Δ 158 ± 5.0 NA 4.30 ± 0.45 0.992 
His6-Sec17 S1 No ATP, +75 nM Qc3Δ 41.0 ± 3.6 NA 5.71 ± 2.23 0.933 
Protein Figure Experimental condition EC50 IC50 Hill coefficient r2 
   nM nM   
Qcwt 2 A No ATP (priming bypass) 3.74 ± 0.66 NA 1.04 ± 0.15 0.979 
Qc7Δ 2 A No ATP (priming bypass) 0.55 ± 0.07 NA 1.03 ± 0.11 0.982 
Qc5Δ 2 A No ATP (priming bypass) 1.05 ± 0.15 NA 0.96 ± 0.11 0.982 
Qc3Δ 2 A No ATP (priming bypass) 4.75 ± 0.53 NA 1.34 ± 0.16 0.989 
Qc5Δ 2 C +ATP (competition with wt) NA 28.9 ± 7.8 −1.10 ± 0.25 0.947 
Qc3Δ 2 C +ATP (competition with wt) NA 40.6 ± 3.4 −1.30 ± 0.12 0.996 
Qc1Δ 2 C and S1 +ATP (competition with wt) NA 656 ± 110 −0.70 ± 0.08 0.733 
QcΔSD 2 C and S1 +ATP (competition with wt) NA 417 ± 45 −0.99 ± 0.10 0.841 
Qc GST-PX S1 +ATP (competition with wt) NA 2,010 ± 247 −0.89 ± 0.09 0.892 
Qcwt 6 A No ATP, +94 nM Sec17 34.3 ± 4.8 NA 0.94 ± 0.08 0.997 
Qc7Δ 6 A No ATP, +94 nM Sec17 8.6 ± 1.1 NA 1.13 ± 0.16 0.990 
Qc5Δ 6 A No ATP, +94 nM Sec17 11.7 ± 1.2 NA 1.33 ± 0.13 0.996 
Qc3Δ 6 A No ATP, +94 nM Sec17 19.9 ± 1.6 NA 1.29 ± 0.07 0.999 
Sec17 wt 6 C No ATP, +75 nM Qc3Δ 125 ± 3.0 NA 4.97 ± 0.46 0.995 
Sec17-LALA 6 C No ATP, +75 nM Qc3Δ 158 ± 5.0 NA 4.30 ± 0.45 0.992 
His6-Sec17 S1 No ATP, +75 nM Qc3Δ 41.0 ± 3.6 NA 5.71 ± 2.23 0.933 

IC50, 50% inhibitory concentration; NA, not applicable; wt, wild type. Errors indicate ±SEM for fits to three or more independently obtained data sets.

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