Percentage of different MHC-typed fibers in EDL and soleus muscles from wild-type and ACTCCo/KO mice
| Age | Muscle | MHC fiber type | Wild type | ACTCCo/KO | P-value |
| mo | % | % | |||
| 4.5 | EDL | MHCIIB | 67.04 ± 2.74 | 61.12 ± 2.97 | 0.124 |
| 4.5 | EDL | MHCIIX | 31.06 ± 2.44 | 40.38 ± 2.44 | 0.014a |
| 4.5 | EDL | MHCIIA | 17.51 ± 1.05 | 19.64 ± 1.59 | 0.091 |
| 10 | EDL | MHCIIB | 71.10 ± 4.07 | 74.85 ± 2.54 | 0.257 |
| 10 | EDL | MHCIIX | 25.12 ± 2.40 | 35.08 ± 3.45 | 0.038a |
| 10 | EDL | MHCIIA | 20.32 ± 4.73 | 13.85 ± 1.80 | 0.537 |
| 4.5 | soleus | MHCI | 46.24 ± 1.21 | 63.54 ± 3.44 | 0.0002b |
| 4.5 | soleus | MHCIIA | 52.91 ± 1.62 | 40.04 ± 3.37 | 0.0021c |
| 10 | soleus | MHCI | 47.03 ± 2.98 | 74.97 ± 3.15 | 0.0095c |
| 10 | soleus | MHCIIA | 49.70 ± 3.20 | 22.75 ± 2.75 | 0.0043c |
| Age | Muscle | MHC fiber type | Wild type | ACTCCo/KO | P-value |
| mo | % | % | |||
| 4.5 | EDL | MHCIIB | 67.04 ± 2.74 | 61.12 ± 2.97 | 0.124 |
| 4.5 | EDL | MHCIIX | 31.06 ± 2.44 | 40.38 ± 2.44 | 0.014a |
| 4.5 | EDL | MHCIIA | 17.51 ± 1.05 | 19.64 ± 1.59 | 0.091 |
| 10 | EDL | MHCIIB | 71.10 ± 4.07 | 74.85 ± 2.54 | 0.257 |
| 10 | EDL | MHCIIX | 25.12 ± 2.40 | 35.08 ± 3.45 | 0.038a |
| 10 | EDL | MHCIIA | 20.32 ± 4.73 | 13.85 ± 1.80 | 0.537 |
| 4.5 | soleus | MHCI | 46.24 ± 1.21 | 63.54 ± 3.44 | 0.0002b |
| 4.5 | soleus | MHCIIA | 52.91 ± 1.62 | 40.04 ± 3.37 | 0.0021c |
| 10 | soleus | MHCI | 47.03 ± 2.98 | 74.97 ± 3.15 | 0.0095c |
| 10 | soleus | MHCIIA | 49.70 ± 3.20 | 22.75 ± 2.75 | 0.0043c |
Muscles were collected from 4.5 (n = 8 or 9)- and 10-mo-old (n = 5) wild-type and ACTCCo/KO mice and stained with anti-MHCI, anti-MHCIIA, anti-MHCIIB, and anti-MHCIIX antibodies. Because of the presence of hybrid fibers expressing more than one MHC type (not depicted), the total percentage of myofibers in the EDL muscles of both types of mice is >100%.
P < 0.05.
P < 0.001.
P < 0.01.