Table IV.

Spine density and length quantification

Description n (neurons); DIV n (spines) Spine density (mean ± SEM) P-value Spine length (mean ± SEM) P-value 
   µm−1  µm  
GFP control 16; 12 749 0.35 ± 0.022  1.22 ± 0.027  
Cdc42 GDP 15; 12 431 0.27 ± 0.027 0.016 1.07 ± 0.067 0.004 
Cdc42 GTP 19; 12 648 0.28 ± 0.022 0.690a 0.95 ± 0.027 0.027a 
    0.020  <0.001 
GFP control 26; 12–13 1,296 0.27 ± 0.023 0.199 1.16 ± 0.018  
Inactive Rif 29; 12–13 994 0.24 ± 0.013  1.10 ± 0.019 0.024 
GFP control 14; 12 749 0.35 ± 0.030  1.10 ± 0.025 0.824 
Active Rif 18; 12 536 0.26 ± 0.018 0.003 1.11 ± 0.033  
GFP control 13; 12 697 0.37 ± 0.031  1.17 ± 0.026 0.292 
Inactive Rif + WA 18; 12 334 0.25 ± 0.023 0.005 1.22 ± 0.046  
GFP control 19; 12–13 995 0.35 ± 0.028  1.16 ± 0.021  
mDia2 siRNA 12; 12–13 385 0.23 ± 0.030 0.046 1.08 ± 0.038 0.027 
GFP control 12; 12 and 14 598 0.41 ± 0.023  1.20 ± 0.031  
Inactive Rif + active mDia2 12; 12 and 14 311 0.26 ± 0.035 0.003 1.07 ± 0.044 0.012 
GFP control 37; 11–13 1,599 0.24 ± 0.018  1.19 ± 0.016  
p34 siRNA 28; 11–13 650 0.17 ± 0.015 0.003 1.40 ± 0.030 <0.001 
W 14; 12 606 0.29 ± 0.025  1.29 ± 0.027 0.444 
WA 15; 12 300 0.16 ± 0.011 <0.001 1.33 ± 0.047  
GFP control 22; 12–13 1,156 0.28 ± 0.026 0.070 1.15 ± 0.019  
cof1 siRNA 23; 12–13 982 0.23 ± 0.012  1.38 ± 0.025 <0.001 
GFP control 17; 12 890 0.29 ± 0.032  1.17 ± 0.021  
cof1 siRNA 18; 12 702 0.21 ± 0.011 0.047 1.37 ± 0.029 <0.001 
cof1 siRNA + cof1 rescue 20; 12 857 0.26 ± 0.019 0.577 1.45 ± 0.024 <0.001 
    0.295b  0.117b 
Description n (neurons); DIV n (spines) Spine density (mean ± SEM) P-value Spine length (mean ± SEM) P-value 
   µm−1  µm  
GFP control 16; 12 749 0.35 ± 0.022  1.22 ± 0.027  
Cdc42 GDP 15; 12 431 0.27 ± 0.027 0.016 1.07 ± 0.067 0.004 
Cdc42 GTP 19; 12 648 0.28 ± 0.022 0.690a 0.95 ± 0.027 0.027a 
    0.020  <0.001 
GFP control 26; 12–13 1,296 0.27 ± 0.023 0.199 1.16 ± 0.018  
Inactive Rif 29; 12–13 994 0.24 ± 0.013  1.10 ± 0.019 0.024 
GFP control 14; 12 749 0.35 ± 0.030  1.10 ± 0.025 0.824 
Active Rif 18; 12 536 0.26 ± 0.018 0.003 1.11 ± 0.033  
GFP control 13; 12 697 0.37 ± 0.031  1.17 ± 0.026 0.292 
Inactive Rif + WA 18; 12 334 0.25 ± 0.023 0.005 1.22 ± 0.046  
GFP control 19; 12–13 995 0.35 ± 0.028  1.16 ± 0.021  
mDia2 siRNA 12; 12–13 385 0.23 ± 0.030 0.046 1.08 ± 0.038 0.027 
GFP control 12; 12 and 14 598 0.41 ± 0.023  1.20 ± 0.031  
Inactive Rif + active mDia2 12; 12 and 14 311 0.26 ± 0.035 0.003 1.07 ± 0.044 0.012 
GFP control 37; 11–13 1,599 0.24 ± 0.018  1.19 ± 0.016  
p34 siRNA 28; 11–13 650 0.17 ± 0.015 0.003 1.40 ± 0.030 <0.001 
W 14; 12 606 0.29 ± 0.025  1.29 ± 0.027 0.444 
WA 15; 12 300 0.16 ± 0.011 <0.001 1.33 ± 0.047  
GFP control 22; 12–13 1,156 0.28 ± 0.026 0.070 1.15 ± 0.019  
cof1 siRNA 23; 12–13 982 0.23 ± 0.012  1.38 ± 0.025 <0.001 
GFP control 17; 12 890 0.29 ± 0.032  1.17 ± 0.021  
cof1 siRNA 18; 12 702 0.21 ± 0.011 0.047 1.37 ± 0.029 <0.001 
cof1 siRNA + cof1 rescue 20; 12 857 0.26 ± 0.019 0.577 1.45 ± 0.024 <0.001 
    0.295b  0.117b 

Statistically significant values (P < 0.050) are shown in bold.

a

P-value for cdc42GDP:cdc42GTP.

b

P-value for cof1 siRNA:cof1 siRNA + cof1 rescue.

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