Table I.

Density of filopodia, thin spines, mushroom spines, and stubby spines

Description n (neurons); DIV Filopodia (mean ± SEM) Thin spines (mean ± SEM) Mushroom spines (mean ± SEM) Stubby spines (mean ± SEM) 
  µm−1 µm−1 µm−1 µm−1 
GFP control 16; 12 0.18 ± 0.016 0.11 ± 0.014 0.038 ± 0.010 0.0096 ± 0.0031 
Cdc42 GDP 15; 12 0.21 ± 0.028 0.017 ± 0.0042 0.013 ± 0.0044 0.018 ± 0.0059 
Cdc42 GTP 17; 12 0.12 ± 0.016 0.024 ± 0.0060 0.039 ± 0.0073 0.054 ± 0.011 
GFP control 16; 12 0.13 ± 0.016 0.090 ± 0.011 0.029 ± 0.0049 0.0097 ± 0.0026 
Inactive Rif 18; 12 0.089 ± 0.0078 0.034 ± 0.0040 0.049 ± 0.0094 0.049 ± 0.0070 
p34 siRNA 12; 12 0.14 ± 0.020 0.015 ± 0.0038 0.018 ± 0.0078 0.0065 ± 0.0017 
cof1 siRNA 16; 12 0.12 ± 0.013 0.043 ± 0.0047 0.025 ± 0.0044 0.0060 ± 0.0016 
cof1 siRNA + cof1-myc 14; 12 0.14 ± 0.017 0.12 ± 0.016 0.024 ± 0.0030 0.011 ± 0.0077 
GFP control 14; 12 0.17 ± 0.016 0.091 ± 0.013 0.035 ± 0.0076 0.0058 ± 0.0019 
Active Rif 18; 12 0.16 ± 0.016 0.058 ± 0.010 0.023 ± 0.0047 0.0056 ± 0.0010 
GFP control 19; 12–13 0.16 ± 0.016 0.11 ± 0.011 0.030 ± 0.0051 0.0078 ± 0.0020 
mDia2 siRNA 12; 12–13 0.11 ± 0.022 0.056 ± 0.013 0.031 ± 0.0046 0.017 ± 0.0040 
GFP control 12; 12, and 14 0.17 ± 0.012 0.16 ± 0.022 0.027 ± 0.0056 0.015 ± 0.0045 
Inactive Rif + active mDia2 12; 12, and 14 0.23 ± 0.032 0.011 ± 0.0070 0.012 ± 0.0059 0.015 ± 0.0091 
GFP control 13; 12 0.18 ± 0.015 0.11 ± 0.014 0.037 ± 0.0057 0.0081 ± 0.0018 
Inactive Rif + WA 18; 12 0.21 ± 0.021 0.019 ± 0.0058 0.0027 ± 0.0015 0.0030 ± 0.0017 
W 14; 12 0.12 ± 0.016 0.093 ± 0.015 0.036 ± 0.0064 0.021 ± 0.0027 
WA 15; 12 0.11 ± 0.015 0.011 ± 0.0025 0.0093 ± 0.0028 0.0068 ± 0.0019 
Description n (neurons); DIV Filopodia (mean ± SEM) Thin spines (mean ± SEM) Mushroom spines (mean ± SEM) Stubby spines (mean ± SEM) 
  µm−1 µm−1 µm−1 µm−1 
GFP control 16; 12 0.18 ± 0.016 0.11 ± 0.014 0.038 ± 0.010 0.0096 ± 0.0031 
Cdc42 GDP 15; 12 0.21 ± 0.028 0.017 ± 0.0042 0.013 ± 0.0044 0.018 ± 0.0059 
Cdc42 GTP 17; 12 0.12 ± 0.016 0.024 ± 0.0060 0.039 ± 0.0073 0.054 ± 0.011 
GFP control 16; 12 0.13 ± 0.016 0.090 ± 0.011 0.029 ± 0.0049 0.0097 ± 0.0026 
Inactive Rif 18; 12 0.089 ± 0.0078 0.034 ± 0.0040 0.049 ± 0.0094 0.049 ± 0.0070 
p34 siRNA 12; 12 0.14 ± 0.020 0.015 ± 0.0038 0.018 ± 0.0078 0.0065 ± 0.0017 
cof1 siRNA 16; 12 0.12 ± 0.013 0.043 ± 0.0047 0.025 ± 0.0044 0.0060 ± 0.0016 
cof1 siRNA + cof1-myc 14; 12 0.14 ± 0.017 0.12 ± 0.016 0.024 ± 0.0030 0.011 ± 0.0077 
GFP control 14; 12 0.17 ± 0.016 0.091 ± 0.013 0.035 ± 0.0076 0.0058 ± 0.0019 
Active Rif 18; 12 0.16 ± 0.016 0.058 ± 0.010 0.023 ± 0.0047 0.0056 ± 0.0010 
GFP control 19; 12–13 0.16 ± 0.016 0.11 ± 0.011 0.030 ± 0.0051 0.0078 ± 0.0020 
mDia2 siRNA 12; 12–13 0.11 ± 0.022 0.056 ± 0.013 0.031 ± 0.0046 0.017 ± 0.0040 
GFP control 12; 12, and 14 0.17 ± 0.012 0.16 ± 0.022 0.027 ± 0.0056 0.015 ± 0.0045 
Inactive Rif + active mDia2 12; 12, and 14 0.23 ± 0.032 0.011 ± 0.0070 0.012 ± 0.0059 0.015 ± 0.0091 
GFP control 13; 12 0.18 ± 0.015 0.11 ± 0.014 0.037 ± 0.0057 0.0081 ± 0.0018 
Inactive Rif + WA 18; 12 0.21 ± 0.021 0.019 ± 0.0058 0.0027 ± 0.0015 0.0030 ± 0.0017 
W 14; 12 0.12 ± 0.016 0.093 ± 0.015 0.036 ± 0.0064 0.021 ± 0.0027 
WA 15; 12 0.11 ± 0.015 0.011 ± 0.0025 0.0093 ± 0.0028 0.0068 ± 0.0019 
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