Summary of translocation data
| Cell type | Primary cilium | PDGF-AA | EIPA | Translocation |
| µm | ||||
| NIH3T3 | No (interphase) | − | − | 60 ± 8.7 (17) |
| NIH3T3 | No (interphase) | − | + | 30 ± 3.0 (25) |
| NIH3T3 | No (interphase) | + | − | 60 ± 5.4 (26) |
| NIH3T3 | No (interphase) | + | + | 33 ± 2.7 (25) |
| NIH3T3 | Yes | − | − | 21 ± 3.6 (23) |
| NIH3T3 | Yes | − | + | 11± 1.8 (23) |
| NIH3T3 | Yes | + | − | 45 ± 5.7 (25) |
| NIH3T3 | Yes | + | + | 13 ± 1.5 (26) |
| WT MEF | Yes | − | − | 45 ± 4.2 (53) |
| WT MEF | Yes | − | + | 27 ± 4.4 (22) |
| WT MEF | Yes | + | − | 111 ± 5.4 (23) |
| WT MEF | Yes | + | + | 15 ± 1.8 (20) |
| Tg737orpk MEF | No or very short | − | − | 75 ± 5.7 (21) |
| Tg737orpk MEF | No or very short | − | + | 63 ± 5.7 (19) |
| Tg737orpk MEF | No or very short | + | − | 87 ± 7.8 (34) |
| Tg737orpk MEF | No or very short | + | + | 57 ± 5.7 (30) |
| Cell type | Primary cilium | PDGF-AA | EIPA | Translocation |
| µm | ||||
| NIH3T3 | No (interphase) | − | − | 60 ± 8.7 (17) |
| NIH3T3 | No (interphase) | − | + | 30 ± 3.0 (25) |
| NIH3T3 | No (interphase) | + | − | 60 ± 5.4 (26) |
| NIH3T3 | No (interphase) | + | + | 33 ± 2.7 (25) |
| NIH3T3 | Yes | − | − | 21 ± 3.6 (23) |
| NIH3T3 | Yes | − | + | 11± 1.8 (23) |
| NIH3T3 | Yes | + | − | 45 ± 5.7 (25) |
| NIH3T3 | Yes | + | + | 13 ± 1.5 (26) |
| WT MEF | Yes | − | − | 45 ± 4.2 (53) |
| WT MEF | Yes | − | + | 27 ± 4.4 (22) |
| WT MEF | Yes | + | − | 111 ± 5.4 (23) |
| WT MEF | Yes | + | + | 15 ± 1.8 (20) |
| Tg737orpk MEF | No or very short | − | − | 75 ± 5.7 (21) |
| Tg737orpk MEF | No or very short | − | + | 63 ± 5.7 (19) |
| Tg737orpk MEF | No or very short | + | − | 87 ± 7.8 (34) |
| Tg737orpk MEF | No or very short | + | + | 57 ± 5.7 (30) |
The table summarizes the translocation data obtained for NIH3T3 cells, WT MEFs, and Tg737orpk MEFs in 5-h wound-healing assays as a function of (a) the presence or absence of a primary cilium, (b) the presence or absence of 50 ng/ml PDGF-AA, and (c) the presence or absence of 10 µM of the NHE1 inhibitor EIPA. The absence or presence of primary cilia was assessed by visual inspection and has also been previously described (Schneider et al., 2005). Translocation data are shown as mean ± SEM , with the number of cells shown in parenthesis.