Quantification of mAb414 immunogold labeling of Nup170p (nup157Δ)-depleted cells
| +Met (h) | Total number of gold particles | Gold particles at NPCs | Gold particles at the inner nuclear membrane |
| 0 | 98 | 82 (84%) | 16 (16%) |
| 4 | 110 | 54 (49%) | 56 (51%) |
| +Met (h) | Total number of gold particles | Gold particles at NPCs | Gold particles at the inner nuclear membrane |
| 0 | 98 | 82 (84%) | 16 (16%) |
| 4 | 110 | 54 (49%) | 56 (51%) |
The +Met (h) column shows time after addition of methionine to the TMY1126 strain. Gold particles present at discontinuities in NE lumen of a size consistent with NPCs were scored as NPC associated. Gold particles at the inner nuclear membrane but distinct from NPCs were scored as inner membrane associated. Percentages of the total number of particles counted are given in parentheses.