Table I.

Distribution of immunogold labeling of SR proteins in the proximal, middle, and distal segments of the BR genes

SR protein/experiment Proximal Middle Distal 
SC35    
 1 0.52 0.29 0.19 
 2 0.48 0.35 0.17 
 3 0.52 0.31 0.17 
 mean (c.i.) 0.51 (0.39–0.66) 0.11 (0.06–0.18) 0.17 (0.13–0.24) 
9G8    
 1 0.53 0.28 0.19 
 2 0.51 0.29 0.20 
 3 0.59 0.27 0.14 
 mean (c.i.) 0.54 (0.35–0.83) 0.09 (0.07–0.12) 0.17 (0.06–0.48) 
ASF/SF2    
 1 0.58 0.29 0.13 
 2 0.62 0.30 0.08 
 3 0.52 0.32 0.16 
 mean (c.i.) 0.57 (0.35–0.94) 0.10 (0.08–0.13) 0.12 (0.02–0.81) 
hrp45/SRp55    
 1 0.30 0.31 0.39 
 2 0.32 0.30 0.38 
 3 0.29 0.33 0.38 
 mean (c.i.) 0.30 (0.25–0.38) 0.10 (0.09–0.13) 0.38 (0.38–0.39) 
SR protein/experiment Proximal Middle Distal 
SC35    
 1 0.52 0.29 0.19 
 2 0.48 0.35 0.17 
 3 0.52 0.31 0.17 
 mean (c.i.) 0.51 (0.39–0.66) 0.11 (0.06–0.18) 0.17 (0.13–0.24) 
9G8    
 1 0.53 0.28 0.19 
 2 0.51 0.29 0.20 
 3 0.59 0.27 0.14 
 mean (c.i.) 0.54 (0.35–0.83) 0.09 (0.07–0.12) 0.17 (0.06–0.48) 
ASF/SF2    
 1 0.58 0.29 0.13 
 2 0.62 0.30 0.08 
 3 0.52 0.32 0.16 
 mean (c.i.) 0.57 (0.35–0.94) 0.10 (0.08–0.13) 0.12 (0.02–0.81) 
hrp45/SRp55    
 1 0.30 0.31 0.39 
 2 0.32 0.30 0.38 
 3 0.29 0.33 0.38 
 mean (c.i.) 0.30 (0.25–0.38) 0.10 (0.09–0.13) 0.38 (0.38–0.39) 

c.i., confidence interval. Three independent experiments are listed for each SR protein. For each experiment, the proportions of gold labeling in the three gene segments are shown. In each experiment, 300–750 gold particles were analyzed for SC35, 9G8, and hrp45/SRp55, and 100–200 gold particles were analyzed for ASF/SF2. The geometric mean and 99% confidence interval for relative distributions of gold labeling/length unit are shown. Data were adjusted for length differences between the proximal, middle, and distal gene segments and were log transformed.

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