The dimensions and proportions of the CENP-A binding domain on human chromosomes
| Sample . | Inner kinetochore length . | Proportion of constriction lengtha . | Inner kinetochore heightb . | Proportion of constriction heightc . | Inner kinetochore widthd . | Proportion of constriction widthe . |
|---|---|---|---|---|---|---|
| nm | nm | nm | ||||
| Acetone fixed (colcemid; n = 9) | 233 ± 16 | 0.68 ± 0.04 | 125 ± 5 | 0.34 ± 0.02 | 102 ± 6 | 0.33 ± 0.02 |
| Acetone fixed (unblocked; n = 4) | 248 ± 18 | 0.74 ± 0.02 | 147 ± 5 | 0.34 ± 0.02 | 85 ± 9 | 0.29 ± 0.04 |
| PFA fixed (n = 5) | 236 ± 9 | 0.62 ± 0.03 | 151 ± 8 | 0.35 ± 0.02 | 96 ± 9 | 0.29 ± 0.02 |
| Sorted mardel(10) (n = 6) | 245 ± 11 | 0.70 ± 0.03 | 135 ± 7 | 0.33 ± 0.01 | 152 ± 12 | 0.31 ± 0.03 |
| Sorted chromosome 18 (n = 5) | 242 ± 28 | 0.66 ± 0.03 | 131 ± 7 | 0.32 ± 0.01 | 148 ± 6 | 0.32 ± 0.02 |
| Sample . | Inner kinetochore length . | Proportion of constriction lengtha . | Inner kinetochore heightb . | Proportion of constriction heightc . | Inner kinetochore widthd . | Proportion of constriction widthe . |
|---|---|---|---|---|---|---|
| nm | nm | nm | ||||
| Acetone fixed (colcemid; n = 9) | 233 ± 16 | 0.68 ± 0.04 | 125 ± 5 | 0.34 ± 0.02 | 102 ± 6 | 0.33 ± 0.02 |
| Acetone fixed (unblocked; n = 4) | 248 ± 18 | 0.74 ± 0.02 | 147 ± 5 | 0.34 ± 0.02 | 85 ± 9 | 0.29 ± 0.04 |
| PFA fixed (n = 5) | 236 ± 9 | 0.62 ± 0.03 | 151 ± 8 | 0.35 ± 0.02 | 96 ± 9 | 0.29 ± 0.02 |
| Sorted mardel(10) (n = 6) | 245 ± 11 | 0.70 ± 0.03 | 135 ± 7 | 0.33 ± 0.01 | 152 ± 12 | 0.31 ± 0.03 |
| Sorted chromosome 18 (n = 5) | 242 ± 28 | 0.66 ± 0.03 | 131 ± 7 | 0.32 ± 0.01 | 148 ± 6 | 0.32 ± 0.02 |
Values are expressed as the mean ± the standard error of the mean.
Calculated as the length of the CENP-A binding domain divided by the length of the constriction.
Calculated as the mean number of sections exhibiting CENP-A labeling multiplied by the mean section thickness.
Calculated as the number of sections exhibiting CENP-A labeling divided by the total number of sections containing the constriction.
The maximum width of labeling observed within a complete set of sections.
Calculated as the maximum kinetochore width divided by the maximum constriction width.