Comparison of actin-dynamic parameters from control and inhibitor-treated epidermal cells
| Control: Growing cells | Nongrowing cells | 100 nM LatB | 20 or 50 mM BDM | |
| Elongation rate; µm/s | 1.7 ± 0.7 (52)a | 1.6 ± 0.8 (57)ND | 0.6 ± 0.2 (51)** | 1.4 ± 0.5 (34)* |
| Severing frequency; breaks/µm/s | 0.011 ± 0.009 (50) | 0.014 ± 0.011 (47)ND | 0.02 ± 0.02 (40)* | 0.004 ± 0.005 (30)** |
| Max. filament length; µm | 14.8 ± 6.4 (78) | 10.3 ± 4.4 (61)** | 4.4 ± 1.6 (57)** | 9.5 ± 5.2 (51)** |
| Filament origin; % de novo/end/side | 25/42/33 (52) | 33/40/26 (57) | 29/55/16 (51) | 18/53/29 (34) |
| Re-growth of severed end; % | 4.4 (362 ends) | 2.1 (290 ends) | 7.7 (154 ends) | 2.5 (122 ends) |
| Convolutedness | 1.6 ± 0.4 (87) | 1.6 ± 0.4 (48)ND | 1.2 ± 0.2 (54)** | 1.5 ± 0.3 (40)ND |
| Rate of change of convolutedness; s−1 | 0.08 ± 0.07 (87) | 0.05 ± 0.03 (48)* | 0.03 ± 0.01 (54)** | 0.02 ± 0.03 (40)** |
| Control: Growing cells | Nongrowing cells | 100 nM LatB | 20 or 50 mM BDM | |
| Elongation rate; µm/s | 1.7 ± 0.7 (52)a | 1.6 ± 0.8 (57)ND | 0.6 ± 0.2 (51)** | 1.4 ± 0.5 (34)* |
| Severing frequency; breaks/µm/s | 0.011 ± 0.009 (50) | 0.014 ± 0.011 (47)ND | 0.02 ± 0.02 (40)* | 0.004 ± 0.005 (30)** |
| Max. filament length; µm | 14.8 ± 6.4 (78) | 10.3 ± 4.4 (61)** | 4.4 ± 1.6 (57)** | 9.5 ± 5.2 (51)** |
| Filament origin; % de novo/end/side | 25/42/33 (52) | 33/40/26 (57) | 29/55/16 (51) | 18/53/29 (34) |
| Re-growth of severed end; % | 4.4 (362 ends) | 2.1 (290 ends) | 7.7 (154 ends) | 2.5 (122 ends) |
| Convolutedness | 1.6 ± 0.4 (87) | 1.6 ± 0.4 (48)ND | 1.2 ± 0.2 (54)** | 1.5 ± 0.3 (40)ND |
| Rate of change of convolutedness; s−1 | 0.08 ± 0.07 (87) | 0.05 ± 0.03 (48)* | 0.03 ± 0.01 (54)** | 0.02 ± 0.03 (40)** |
ND, Not significantly different from control value by Student's t test; P value >0.05.
*, Significantly different from control value by Student's t test; P value ≤0.02.
**, Significantly different from control value by Student's t test; P value <0.0001.
Values given are means ± SD, with (n) = number of filaments in parentheses. See text for details of experimental treatment and measurement.