Synthetic oligonucleotide primers used
| Code | Sequence (5′ to 3′) | Underlined (nucleotide sequence at) |
|---|---|---|
| 3F | CCCAAGCTTATGCTGAGATCAATGTGGAAT | 1–21 of rat PEX3 |
| 3R | CGCGGATCCTTTCTCCAGTTGTTGGGGGGT | 1096–1116 of rat PEX3 |
| 3(1–50)R | CGCGGATCCGGCAATGTACTCTGCAGCTTC | 130–150 of rat PEX3 |
| 16HA2F | GCTGGATCCATGGCGTACCCCTACGATGTGCCCGATTATGCATACCCCTACGATGTGCCCGATTATGCAATGGAGA AGCTGCGGCTCCTGGGC | Tandem HA (tagging to human PEX16) |
| 16R | GCTCTAGATCAGCCCCAACTGTAGAAGTA | 991–1000 plus a stop codon of human PEX16 |
| 11αHA2F | GCTGGATCCATGGCGTACCCCTACGATGTGCCCGATTATGCATACCCCTACGATGTGCCCGATTATGCAATGGACG CCTTCACCCGCTTCACC | Tandem HA (tagging to human PEX11α) |
| 11αR | GCTCTAGACTAACGGGTCTTCAGCTTCAT | 724–741 plus a stop codon of human PEX11α |
| SSF | CGGGGTACCGCCATGGGAGCTGCTGCAAAGTTGGCGTTCGCCGTCTTTCTTATCTCTTGCTCTTCAGGTGCTATACTTGGCAGATACCCATACGATGTTCCAGATTACGCTGGATCC ATGGCG | Signal sequence of mouse activin type IIA receptor |
| m16R | GCTCAGCCATGTCAGCAGCTTCTGCTGGGACAGCGACAC TGGGAGTTTCTTTCTCAATTCTTTCCGTAGGATCCCGTCA TTGAG | A silence mutation in human PEX16 |
| m16F | AAGCTGCTGACATGGCTGAGCGTGCTGGAGTGCGTGGAAGTCTTTATGGAAATGGGCGCT | A silence mutation in human PEX16 |
| Code | Sequence (5′ to 3′) | Underlined (nucleotide sequence at) |
|---|---|---|
| 3F | CCCAAGCTT | 1–21 of rat |
| 3R | CGCGGATCC | 1096–1116 of rat |
| 3(1–50)R | CGCGGATCC | 130–150 of rat |
| 16HA2F | GCTGGATCCATGGCG | Tandem |
| 16R | GCTCTAGA | 991–1000 plus a stop codon of human |
| 11αHA2F | GCTGGATCCATGGCG | Tandem |
| 11αR | GCTCTAGA | 724–741 plus a stop codon of human |
| SSF | CGGGGTACCGCC | Signal sequence of mouse activin type IIA receptor |
| m16R | GCTCAGCCATGTCAGCAGCTTCTGCTGGGACAGCGACAC | A silence mutation in human |
| m16F | AAGCTGCTGACATGGCTGAGCGTGCTGGAGTGCGTG | A silence mutation in human |
F and R, forward and reverse primers, respectively.