Table I.
Summary of all transgenic constructs presented in this study
| Construct | Cartoon | Primary localization | Figure |
|---|---|---|---|
| GFP |  | IS, soluble | Fig. 5 A |
| GFP-R9AP |  | OS | Fig. 3 A and Fig. 10 |
| GFP-R9APΔHabc |  | OS | Fig. 3 B |
| GFP-R9APΔSH |  | OS | Fig. 3 C |
| GFP-R9AP TM |  | OS | Fig. 3 D and Fig. 9 A |
| GFP-R9APΔTM |  | IS, soluble | Fig. 3 E |
| GFP-R9AP TM shorter (−2 aa) |  | OS and IS | Fig. 9 B |
| GFP-R9AP TM shortest (−4 aa) |  | IS | Fig. 9 C |
| Myc-R9AP |  | OS | Fig. 6 A and Fig. S1 A |
| Myc-R9AP TM shorter (−2 aa) |  | OS and IS | Fig. S3 |
| Myc-R9APΔHabc |  | OS | Fig. S1 B |
| Myc-R9APΔSH |  | OS | Fig. S1 C |
| GFP-mGluR1 TM 1 |  | OS | Fig. 7 |
| GFP-Cyt b5 TM |  | ER | Fig. 8 A and Fig. S2 |
| GFP-Cyt b5 TM longer (+4 aa) |  | OS | Fig. 8 B |
| GFP-BclXL TM |  | MT | Fig. 8 C |
| GFP-BclXL TM K233S |  | OS and IS | Fig. 8 D |
| GFP-BclXL TM, K233S, longer (+4 aa) |  | OS | Fig. 8 E |
| GFP-Stx 3 |  | ISPM | Fig. 4 A |
| GFP-Stx 3(FMDE-AAAA) |  | ISPM | Fig. 4 E |
| GFP-Stx 3ΔHabc |  | ISPM and IS | Fig. 4 B |
| GFP-Stx 3ΔSH |  | OS and ISPM | Fig. 4 C |
| GFP-Stx 3 TM |  | OS | Fig. 4 D and Fig. 10 |
| GFP-Stx 3ΔTM |  | IS, soluble | Fig. 5 C |
| GFP-Stx 3ΔTM gg |  | ISPM | Fig. 5 F |
| GFP-Stx 3 Habc |  | IS, soluble | Fig. 5 B |
| GFP-Stx 3 Habc gg |  | OS | Fig. 5 E |
| Myc-Stx 3 |  | ISPM | Fig. 6 B |
| Myc-R9AP/Stx 3(TM) |  | OS | Fig. 6 C |
| Myc-Stx 3/R9AP(SH) |  | OS | Fig. 6 D |
| Myc-Stx 3/R9AP(TM) |  | OS | Fig. 6 E |
| Myc-R9AP/Stx 3(SH) |  | IS | Fig. 6 F |
| Construct | Cartoon | Primary localization | Figure |
|---|---|---|---|
| GFP | | IS, soluble | Fig. 5 A |
| GFP-R9AP | | OS | Fig. 3 A and Fig. 10 |
| GFP-R9APΔHabc | | OS | Fig. 3 B |
| GFP-R9APΔSH | | OS | Fig. 3 C |
| GFP-R9AP TM | | OS | Fig. 3 D and Fig. 9 A |
| GFP-R9APΔTM | | IS, soluble | Fig. 3 E |
| GFP-R9AP TM shorter (−2 aa) | | OS and IS | Fig. 9 B |
| GFP-R9AP TM shortest (−4 aa) | | IS | Fig. 9 C |
| Myc-R9AP | | OS | Fig. 6 A and Fig. S1 A |
| Myc-R9AP TM shorter (−2 aa) | | OS and IS | Fig. S3 |
| Myc-R9APΔHabc | | OS | Fig. S1 B |
| Myc-R9APΔSH | | OS | Fig. S1 C |
| GFP-mGluR1 TM 1 | | OS | Fig. 7 |
| GFP-Cyt b5 TM | | ER | Fig. 8 A and Fig. S2 |
| GFP-Cyt b5 TM longer (+4 aa) | | OS | Fig. 8 B |
| GFP-BclXL TM | | MT | Fig. 8 C |
| GFP-BclXL TM K233S | | OS and IS | Fig. 8 D |
| GFP-BclXL TM, K233S, longer (+4 aa) | | OS | Fig. 8 E |
| GFP-Stx 3 | | ISPM | Fig. 4 A |
| GFP-Stx 3(FMDE-AAAA) | | ISPM | Fig. 4 E |
| GFP-Stx 3ΔHabc | | ISPM and IS | Fig. 4 B |
| GFP-Stx 3ΔSH | | OS and ISPM | Fig. 4 C |
| GFP-Stx 3 TM | | OS | Fig. 4 D and Fig. 10 |
| GFP-Stx 3ΔTM | | IS, soluble | Fig. 5 C |
| GFP-Stx 3ΔTM gg | | ISPM | Fig. 5 F |
| GFP-Stx 3 Habc | | IS, soluble | Fig. 5 B |
| GFP-Stx 3 Habc gg | | OS | Fig. 5 E |
| Myc-Stx 3 | | ISPM | Fig. 6 B |
| Myc-R9AP/Stx 3(TM) | | OS | Fig. 6 C |
| Myc-Stx 3/R9AP(SH) | | OS | Fig. 6 D |
| Myc-Stx 3/R9AP(TM) | | OS | Fig. 6 E |
| Myc-R9AP/Stx 3(SH) | | IS | Fig. 6 F |
ER, endoplasmic reticulum; gg, geranylgeranylated; IS, inner segment; ISPM; inner segment plasma membrane, MT, mitochondria; OS, outer segment.