Table I.

Kinase inhibitor screening

KinasesInhibitors

100 μM Genistein
10 μM PP2
1 μM SU6656
10 μM Imatinib
200 μM LFM-A13
1 μM VEGFR kinase inhibitor
ABL −   
ABL1 E255K     
ABL1 G250E     
ABL1 Y253F     
ABL2 (ARG)     
BMX   −  
CSK  − −   
EGFR − − −  
ERBB4 (HER4)     
FGR     
FLT4 (VEGFR3)   −  
FYN −   
KDR (VEGFR2)   −  
PDGFRA D842V     
PDGFRB  −  
SRC −  − 
YES −   
KinasesInhibitors

100 μM Genistein
10 μM PP2
1 μM SU6656
10 μM Imatinib
200 μM LFM-A13
1 μM VEGFR kinase inhibitor
ABL −   
ABL1 E255K     
ABL1 G250E     
ABL1 Y253F     
ABL2 (ARG)     
BMX   −  
CSK  − −   
EGFR − − −  
ERBB4 (HER4)     
FGR     
FLT4 (VEGFR3)   −  
FYN −   
KDR (VEGFR2)   −  
PDGFRA D842V     
PDGFRB  −  
SRC −  − 
YES −   

Listed are tyrosine kinases that are PP2 sensitive, expressed in arterial ECs, and likely localized to cell–cell contacts. Their susceptibility to other inhibitors is indicated. −, inhibition by <60% ; +, inhibition by >60%; blank, no data available. PECAM-1 response was inhibited by Genistein, PP2, and SU6656 and was not inhibited by Imatinib, LFM-A13, and VEGFR kinase inhibitor (Fig. 5, A and B). Kinase susceptibility is obtained from the following sources: Genistein, Akiyama and Ogawara (1991); PP2 and Imatinib, http://www.invitrogen.com/downloads/SelectScreen_Data_193.pdf; SU6656, Blake et al. (2000); LFM-A13, Chau et al. (2002) and Mahajan et al. (1999); and VEGFR kinase inhibitor, Cools et al. (2004) and Fraley et al. (2002).

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