Parameters that define tight junction protein dynamic behavior in computer simulations
| Tight junction protein . | Value . |
|---|---|
| Occludin | |
| Tight junction fraction (all mobile) | 80% |
| Lateral membrane fraction (all mobile) | 20% |
| Intracellular fraction | 0% |
| Tight junction diffusion constant | 0.011 μm2s−1 |
| Lateral membrane diffusion constant | 0.1 μm2s−1 |
| Rate constant for movement to tight junction (Kf) | 0.0040 s−1 |
| Rate constant for movement from tight junction (Kr) | 0.0010 s−1 |
| Claudin-1 | |
| Tight junction fraction | 100% |
| Immobile fraction at the tight junction | 60% |
| Mobile fraction at the tight junction | 40% |
| Lateral membrane fraction | 0% |
| Intracellular fraction | 0% |
| Tight junction diffusion constant, immobile fraction | 0 μm2s−1 |
| Tight junction diffusion constant, mobile fraction | 0.011 μm2s−1 |
| ZO-1 | |
| Tight junction fraction | 40% |
| Exchangeable fraction at the tight junction | 25% |
| Nonexchangeable fraction at the tight junction | 15% |
| Lateral membrane fraction | 0% |
| Intracellular fraction | 60% |
| Tight junction diffusion constant, exchangeable and nonexchangeable fractions | 0 μm2s−1 |
| Intracellular diffusion constant | 1 μm2s−1 |
| Rate constant for movement to tight junction (Kf), exchangeable fraction | 0.25 μm2s−1 |
| Rate constant for movement from tight junction (Kr), exchangeable fraction | 0.0075 s−1 |
| Rate constant for movement to tight junction (Kf), nonexchangeable fraction | 0 μm2s−1 |
| Rate constant for movement from tight junction (Kr), nonexchangeable fraction | 0 s−1 |
| Tight junction protein . | Value . |
|---|---|
| Occludin | |
| Tight junction fraction (all mobile) | 80% |
| Lateral membrane fraction (all mobile) | 20% |
| Intracellular fraction | 0% |
| Tight junction diffusion constant | 0.011 μm2s−1 |
| Lateral membrane diffusion constant | 0.1 μm2s−1 |
| Rate constant for movement to tight junction (Kf) | 0.0040 s−1 |
| Rate constant for movement from tight junction (Kr) | 0.0010 s−1 |
| Claudin-1 | |
| Tight junction fraction | 100% |
| Immobile fraction at the tight junction | 60% |
| Mobile fraction at the tight junction | 40% |
| Lateral membrane fraction | 0% |
| Intracellular fraction | 0% |
| Tight junction diffusion constant, immobile fraction | 0 μm2s−1 |
| Tight junction diffusion constant, mobile fraction | 0.011 μm2s−1 |
| ZO-1 | |
| Tight junction fraction | 40% |
| Exchangeable fraction at the tight junction | 25% |
| Nonexchangeable fraction at the tight junction | 15% |
| Lateral membrane fraction | 0% |
| Intracellular fraction | 60% |
| Tight junction diffusion constant, exchangeable and nonexchangeable fractions | 0 μm2s−1 |
| Intracellular diffusion constant | 1 μm2s−1 |
| Rate constant for movement to tight junction (Kf), exchangeable fraction | 0.25 μm2s−1 |
| Rate constant for movement from tight junction (Kr), exchangeable fraction | 0.0075 s−1 |
| Rate constant for movement to tight junction (Kf), nonexchangeable fraction | 0 μm2s−1 |
| Rate constant for movement from tight junction (Kr), nonexchangeable fraction | 0 s−1 |