The dynamic behavior of tight junction proteins changes after confluence
. | Postconfluent time . | Mobile fraction . | t1/2 . |
|---|---|---|---|
| d | % | s | |
| Occludin | |||
| 1 | 74 ± 7 | 139 ± 17 | |
| 3 | 71 ± 3 | 194 ± 19 | |
| 10 | 77 ± 3 | 107 ± 8a | |
| Claudin-1 | |||
| 1 | 25 ± 3 | 142 ± 36 | |
| 3 | 24 ± 5 | 200 ± 21 | |
| 10 | 22 ± 2 | 195 ± 65 | |
| ZO-1 | |||
| 1 | 87 ± 5a | 101 ±1 5 | |
| 3 | 69 ± 5 | 119 ± 21 | |
| 10 | 72 ± 3 | 98 ± 16 | |
| β-Actin | |||
| 1 | 99 ± 2 | 15 ± 1a | |
| 3 | 99 ± 6 | 106 ± 18 | |
| 10 | 97 ± 3 | 43 ± 8a |
. | Postconfluent time . | Mobile fraction . | t1/2 . |
|---|---|---|---|
| d | % | s | |
| Occludin | |||
| 1 | 74 ± 7 | 139 ± 17 | |
| 3 | 71 ± 3 | 194 ± 19 | |
| 10 | 77 ± 3 | 107 ± 8a | |
| Claudin-1 | |||
| 1 | 25 ± 3 | 142 ± 36 | |
| 3 | 24 ± 5 | 200 ± 21 | |
| 10 | 22 ± 2 | 195 ± 65 | |
| ZO-1 | |||
| 1 | 87 ± 5a | 101 ±1 5 | |
| 3 | 69 ± 5 | 119 ± 21 | |
| 10 | 72 ± 3 | 98 ± 16 | |
| β-Actin | |||
| 1 | 99 ± 2 | 15 ± 1a | |
| 3 | 99 ± 6 | 106 ± 18 | |
| 10 | 97 ± 3 | 43 ± 8a |
All measurements represent means of at least five independent measurements.
P < 0.05 versus 3-d monolayers.