Telomeric fusions caused by expression of TIN2 mutants
| Lentiviral vector . | No. metaphases analyzed . | No. chromosome fusions . | . | Fusions per metaphase . | Fusions per chromosome . | |
|---|---|---|---|---|---|---|
. | . | +Tel . | –Tel . | . | . | |
| GFP | 53 | 0 | 0 | 0 | 0 | |
| TIN2-13 | 53 | 7 | 3 | 0.18 | 0.0034 | |
| TIN2-15C | 61 | 42 | 10 | 0.85 | 0.0175 | |
| Lentiviral vector . | No. metaphases analyzed . | No. chromosome fusions . | . | Fusions per metaphase . | Fusions per chromosome . | |
|---|---|---|---|---|---|---|
. | . | +Tel . | –Tel . | . | . | |
| GFP | 53 | 0 | 0 | 0 | 0 | |
| TIN2-13 | 53 | 7 | 3 | 0.18 | 0.0034 | |
| TIN2-15C | 61 | 42 | 10 | 0.85 | 0.0175 | |
Presenescent HCA2 cells were infected with a retroviruses expressing GSE-22, selected, and infected with lentiviruses expressing GFP, TIN2-13, or TIN2-15C. The cells were treated with colcemid, and telomeres on metaphase chromosomes were identified by PNA-FISH as described in Materials and methods. Chromosome fusions were scored by fluorescence in situ hybridization for the presence (+Tel) or absence (−Tel) of telomeric DNA at the site of fusion.