Dynamics of MTs assembled with FH1FH2mDia2 or buffer alone
. | Plus ends . | Minus ends . |
|---|---|---|
| Buffer | Ve = 1.82 ± 0.54 (25) | Ve = 0.99 ± 0.23 (21) |
| Te = 0.95 ± 0.59 (25) | Te = 0.96 ± 0.57 (21) | |
| Vs = 30.20 ± 7.90 (11) | Vs = 27.67 ± 11.76 (3) | |
| Ts = 0.21 ± 0.09 (11) | Ts = 0.17 ± 0.06 (3) | |
| Cf = 0.44 ± 0.13 (11) | Cf = 0.14 ± 0.08 (3) | |
| mDia2 | Ve = 1.42 ± 0.47 (30)** | Ve = 0.75 ± 0.45 (40)* |
| Te = 0.97 ± 0.62 (30) | Te = 1 ± 0.58 (40) | |
| Vs = 35.57 ± 14.78 (8) | Vs = 28.73 ± 26.07 (6) | |
| Ts = 0.15 ± 0.07 (8) | Ts = 0.25 ± 0.22 (6) | |
| Cf = 0.27 ± 0.09 (8) | Cf = 0.14 ± 0.05 (6) |
. | Plus ends . | Minus ends . |
|---|---|---|
| Buffer | Ve = 1.82 ± 0.54 (25) | Ve = 0.99 ± 0.23 (21) |
| Te = 0.95 ± 0.59 (25) | Te = 0.96 ± 0.57 (21) | |
| Vs = 30.20 ± 7.90 (11) | Vs = 27.67 ± 11.76 (3) | |
| Ts = 0.21 ± 0.09 (11) | Ts = 0.17 ± 0.06 (3) | |
| Cf = 0.44 ± 0.13 (11) | Cf = 0.14 ± 0.08 (3) | |
| mDia2 | Ve = 1.42 ± 0.47 (30)** | Ve = 0.75 ± 0.45 (40)* |
| Te = 0.97 ± 0.62 (30) | Te = 1 ± 0.58 (40) | |
| Vs = 35.57 ± 14.78 (8) | Vs = 28.73 ± 26.07 (6) | |
| Ts = 0.15 ± 0.07 (8) | Ts = 0.25 ± 0.22 (6) | |
| Cf = 0.27 ± 0.09 (8) | Cf = 0.14 ± 0.05 (6) |
Dynamics obtained with axonemal fragments using 10 μM PC tubulin and 2 μM His-FH1FH2mDia2 (mDia2) or buffer alone. Values are mean ± SD with the number of MTs observed indicated in parentheses. Ve, elongation rate (μm/min); Te, time spent in elongation (min); Vs, shortening rate (μm/min); Ts, time spent in shortening (min); Cf, catastrophe frequency (min−1). *, P < 0.05; **, P < 0.01 (Student's t test).