Summary of mobile fractions of various ER molecules after hypertonicity treatment
| . | +Sorbitol . | . | . | ||
|---|---|---|---|---|---|
. | 0 min . | 1 min . | 60 min . | ||
| % | % | % | |||
| Cargo and ER-resident proteins | |||||
| VSVG (40°C) | 93.4 (12) | 10.8 (12) | 17.8 (12) | ||
| VSVG (32°C) | 97.5 (8) | 15.7 (8) | 9.5 (8) | ||
| VSVG(N336S) | 86.9 (10) | 60.3 (5) | 62.8 (8) | ||
| VSVG (null) | 87.6 (5) | 69.7 (5) | 71.1 (5) | ||
| Tyrosinase | 100.8 (8) | 7.2 (12) | 19.4 (12) | ||
| α1ATNHK | 89.3 (9) | 50.1 (8) | 27.1 (8) | ||
| KDELR | 91.8 (8) | 81.5 (8) | 82.8 (8) | ||
| Esterase | 72.9 (4) | 53.8 (4) | 70.2 (7) | ||
| Ero1L | 77.6 (5) | 64.7 (3) | 76.1 (4) | ||
| BiP | 81.5 (5) | 52.0 (6) | 81.7 (16) | ||
| Lectin-related proteins | |||||
| Calnexin | 89.6 (17) | 44.0 (18) | 86.6 (13) | ||
| Calnexin(Y163F) | 82.4 (6) | 66.3 (3) | 79.7 (5) | ||
| ERGIC53 | 77.7 (5) | 49.3 (4) | 68.7 (9) | ||
| ERGIC53(N516A) | 74.3 (4) | 50.7 (3) | 69.4 (6) | ||
| VIP36 | 102.9 (3) | 88.9 (3) | 84.6 (6) | ||
| VIP36(N166A) | 96.1 (5) | 77.0 (3) | 94.1 (6) | ||
| VIPL | 95.2 (9) | 18.1 (8) | 18.1 (7) | ||
| VIPL(N163A) | 82.5 (5) | 44.8 (3) | 78.5 (6) | ||
| UGGT | 88.8 (3) | 31.2 (3) | 69.2 (5) | ||
| Artificial cargo | |||||
| DiOC6 | 108.4 (12) | 94.3 (12) | 95.6 (12) | ||
| YE | 95.8 (7) | 99.3 (7) | 97.9 (7) | ||
| YE(gly)1 | 88.1 (4) | 77.9 (4) | 87.5 (4) | ||
| YE(gly)2 | 98.0 (9) | 32.8 (9) | 53.3 (9) | ||
| Y2E | 81.8 (9) | 71.3 (4) | 87.7 (5) | ||
| . | +Sorbitol . | . | . | ||
|---|---|---|---|---|---|
. | 0 min . | 1 min . | 60 min . | ||
| % | % | % | |||
| Cargo and ER-resident proteins | |||||
| VSVG (40°C) | 93.4 (12) | 10.8 (12) | 17.8 (12) | ||
| VSVG (32°C) | 97.5 (8) | 15.7 (8) | 9.5 (8) | ||
| VSVG(N336S) | 86.9 (10) | 60.3 (5) | 62.8 (8) | ||
| VSVG (null) | 87.6 (5) | 69.7 (5) | 71.1 (5) | ||
| Tyrosinase | 100.8 (8) | 7.2 (12) | 19.4 (12) | ||
| α1ATNHK | 89.3 (9) | 50.1 (8) | 27.1 (8) | ||
| KDELR | 91.8 (8) | 81.5 (8) | 82.8 (8) | ||
| Esterase | 72.9 (4) | 53.8 (4) | 70.2 (7) | ||
| Ero1L | 77.6 (5) | 64.7 (3) | 76.1 (4) | ||
| BiP | 81.5 (5) | 52.0 (6) | 81.7 (16) | ||
| Lectin-related proteins | |||||
| Calnexin | 89.6 (17) | 44.0 (18) | 86.6 (13) | ||
| Calnexin(Y163F) | 82.4 (6) | 66.3 (3) | 79.7 (5) | ||
| ERGIC53 | 77.7 (5) | 49.3 (4) | 68.7 (9) | ||
| ERGIC53(N516A) | 74.3 (4) | 50.7 (3) | 69.4 (6) | ||
| VIP36 | 102.9 (3) | 88.9 (3) | 84.6 (6) | ||
| VIP36(N166A) | 96.1 (5) | 77.0 (3) | 94.1 (6) | ||
| VIPL | 95.2 (9) | 18.1 (8) | 18.1 (7) | ||
| VIPL(N163A) | 82.5 (5) | 44.8 (3) | 78.5 (6) | ||
| UGGT | 88.8 (3) | 31.2 (3) | 69.2 (5) | ||
| Artificial cargo | |||||
| DiOC6 | 108.4 (12) | 94.3 (12) | 95.6 (12) | ||
| YE | 95.8 (7) | 99.3 (7) | 97.9 (7) | ||
| YE(gly)1 | 88.1 (4) | 77.9 (4) | 87.5 (4) | ||
| YE(gly)2 | 98.0 (9) | 32.8 (9) | 53.3 (9) | ||
| Y2E | 81.8 (9) | 71.3 (4) | 87.7 (5) | ||
n is given in parentheses for the number of measurements. Mobile fractions of various molecules in the ER before and after osmotic stress were estimated. All molecules except DiOC6 were fused to YFP.