Summary of the phenotypic characterization of the sec3 mutants
| . | sec3 single mutants . | . | . | sec3 exo70-38 double mutants . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
. | Mutation sites . | Exocyst localization . | Lat B treatment . | Synthetic effects . | Exocyst localization . | Vesicle accumulation . | ||||
| SEC3 | N/A | Polarized | 66% polarized | None | Polarized | 100 ± 38 (35°C) (in exo70-38 single mutant) | ||||
| sec3-201 | I140A, L141A, S142A, P145A | Polarized | 25.7% polarized | Inviable >32°C | Depolarized | 366 ± 80 (35°C) | ||||
| sec3-202 | K134A, K135A, K136A, R137A | Polarized | 31.9% polarized | Inviable >35°C | Depolarized | ND | ||||
| sec3-203 | K134E, K135E, K136E, R137E | Polarized | 27.9% polarized | Inviable >33°C | Depolarized | 330 ± 69 (35°C) | ||||
| sec3-204 | K134A, K135A, K136A, R137A, I140A, L141A, S142A, P145A. | Polarized | 11.7% polarized | Synthetic lethal | ND | ND | ||||
| sec3-205 | K134E, K135E, K136E, R137E, I140A, L141A, S142A, P145A | Polarized | 6.8% polarized | Synthetic lethal | ND | ND | ||||
| . | sec3 single mutants . | . | . | sec3 exo70-38 double mutants . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
. | Mutation sites . | Exocyst localization . | Lat B treatment . | Synthetic effects . | Exocyst localization . | Vesicle accumulation . | ||||
| SEC3 | N/A | Polarized | 66% polarized | None | Polarized | 100 ± 38 (35°C) (in exo70-38 single mutant) | ||||
| sec3-201 | I140A, L141A, S142A, P145A | Polarized | 25.7% polarized | Inviable >32°C | Depolarized | 366 ± 80 (35°C) | ||||
| sec3-202 | K134A, K135A, K136A, R137A | Polarized | 31.9% polarized | Inviable >35°C | Depolarized | ND | ||||
| sec3-203 | K134E, K135E, K136E, R137E | Polarized | 27.9% polarized | Inviable >33°C | Depolarized | 330 ± 69 (35°C) | ||||
| sec3-204 | K134A, K135A, K136A, R137A, I140A, L141A, S142A, P145A. | Polarized | 11.7% polarized | Synthetic lethal | ND | ND | ||||
| sec3-205 | K134E, K135E, K136E, R137E, I140A, L141A, S142A, P145A | Polarized | 6.8% polarized | Synthetic lethal | ND | ND | ||||