Fragile site expression in wild-type and WRN-deficient (WRNi) cells
| Cell line | Treatment | Mean gaps and breaks per cell | Percentage of FRA7H loci with a break | Percentage of total breaks attributable to FRA7H | Percentage of FRA716D loci with a break | Percentage of total breaks attributable to FRA16D |
|---|---|---|---|---|---|---|
| Wild type | −APH | 0.5 | 0 | 0 | 0 | 0 |
| +APH | 0.6 | 1 | 3.3 | 1.1 | 3.6 | |
| WRNi | −APH | 1.3 | 8 | 12.3 | 7 | 10.7 |
| +APH | 2.2 | 16 | 14.5 | 13 | 11.8 |
| Cell line | Treatment | Mean gaps and breaks per cell | Percentage of FRA7H loci with a break | Percentage of total breaks attributable to FRA7H | Percentage of FRA716D loci with a break | Percentage of total breaks attributable to FRA16D |
|---|---|---|---|---|---|---|
| Wild type | −APH | 0.5 | 0 | 0 | 0 | 0 |
| +APH | 0.6 | 1 | 3.3 | 1.1 | 3.6 | |
| WRNi | −APH | 1.3 | 8 | 12.3 | 7 | 10.7 |
| +APH | 2.2 | 16 | 14.5 | 13 | 11.8 |
APH, 0.05 μM aphidicolin.