Thin filament length parameters determined by distributed deconvolution analysis of fluorescence images
| Muscle type | Phalloidin | Nebulin M1M2M3 | Tmod | ||||||
| Mean ± SD | Min–max | n | Mean ± SD | Min–max | n | Mean ± SD | Min–max | n | |
| µm | µm | µm | µm | µm | µm | ||||
| Embryonic flank | |||||||||
| Tmod1+/+ | 1.18 ± 0.09 | 1.02–1.35 | 122 | 0.86 ± 0.04 | 0.77–0.98 | 57 | 1.23 ± 0.10 | 1.09–1.50 | 27 |
| Tmod1−/−Tg+ | 1.19 ± 0.06 | 1.07–1.36 | 100 | 0.85 ± 0.04 | 0.75–0.97 | 94 | 1.26 ± 0.07 | 1.13–1.40 | 43 |
| Adult EDL | |||||||||
| Tmod1+/+ | 1.06 ± 0.07 | 0.92–1.25 | 79 | 0.84 ± 0.07 | 0.68–1.06 | 50 | 1.11 ± 0.06 | 0.92–1.27 | 67 |
| Tmod1−/−Tg+ | 1.07 ± 0.06 | 0.94–1.21 | 67 | 0.85 ± 0.04 | 0.75–1.00 | 61 | 1.13 ± 0.09 | 0.89–1.33 | 82 |
| Adult soleus | |||||||||
| Tmod1+/+ | 1.15 ± 0.07 | 0.95–1.29 | 86 | 0.90 ± 0.05 | 0.83–1.03 | 50 | 1.22 ± 0.08 | 1.02–1.34 | 42 |
| Tmod1−/−Tg+ | 1.16 ± 0.07 | 1.00–1.31 | 114 | 0.89 ± 0.03 | 0.81–0.96 | 52 | 1.22 ± 0.06 | 1.11–1.37 | 51 |
| Muscle type | Phalloidin | Nebulin M1M2M3 | Tmod | ||||||
| Mean ± SD | Min–max | n | Mean ± SD | Min–max | n | Mean ± SD | Min–max | n | |
| µm | µm | µm | µm | µm | µm | ||||
| Embryonic flank | |||||||||
| Tmod1+/+ | 1.18 ± 0.09 | 1.02–1.35 | 122 | 0.86 ± 0.04 | 0.77–0.98 | 57 | 1.23 ± 0.10 | 1.09–1.50 | 27 |
| Tmod1−/−Tg+ | 1.19 ± 0.06 | 1.07–1.36 | 100 | 0.85 ± 0.04 | 0.75–0.97 | 94 | 1.26 ± 0.07 | 1.13–1.40 | 43 |
| Adult EDL | |||||||||
| Tmod1+/+ | 1.06 ± 0.07 | 0.92–1.25 | 79 | 0.84 ± 0.07 | 0.68–1.06 | 50 | 1.11 ± 0.06 | 0.92–1.27 | 67 |
| Tmod1−/−Tg+ | 1.07 ± 0.06 | 0.94–1.21 | 67 | 0.85 ± 0.04 | 0.75–1.00 | 61 | 1.13 ± 0.09 | 0.89–1.33 | 82 |
| Adult soleus | |||||||||
| Tmod1+/+ | 1.15 ± 0.07 | 0.95–1.29 | 86 | 0.90 ± 0.05 | 0.83–1.03 | 50 | 1.22 ± 0.08 | 1.02–1.34 | 42 |
| Tmod1−/−Tg+ | 1.16 ± 0.07 | 1.00–1.31 | 114 | 0.89 ± 0.03 | 0.81–0.96 | 52 | 1.22 ± 0.06 | 1.11–1.37 | 51 |
Min, minimum; max, maximum. All lengths are in micrometers from the Z-line, measured from n myofibrils from three to four mice per genotype. All nebulin M1M2M3 lengths were significantly closer to the Z-line than corresponding Tmod lengths (P < 10−38). For embryonic flank phalloidin, nebulin M1M2M3, and Tmod, P = 0.08, P = 0.64, and P = 0.14, respectively. For adult EDL phalloidin, nebulin M1M2M3, and Tmod, P = 0.36, P = 0.48, and P = 0.20, respectively. For adult soleus phalloidin, nebulin M1M2M3, and Tmod, P = 0.39, P = 0.16, and P = 0.87, respectively.