Characteristics of transgenic mice
| Transgenea | Expression levelb | CtmPrP in vitroc | CtmPrP in cells | CtmPrP in vivod | Time to disease |
| % | % | d | |||
| PrP(A3922) | 4.0× | ∼10 | <2 | <2 | >700 |
| PrP(KH-II)H | 3.0× | ∼30 | ∼10 | ∼10–20 | ∼60 |
| PrP(KH-II)M | 0.6× | ∼30 | ∼10 | ∼5–8 | ∼470 |
| PrP(A117V)H | 4.0× | ∼15 | ∼4 | ∼5–8 | ∼570 |
| PrP(AV3) | 1–4× | ∼35 | ∼10 | ∼10–20 | <60 |
| Prl-PrP(AV3)6 | 4.7× | ∼15 | <2 | 2–5 | >600 |
| Prl-PrP(AV3)10 | 2.4× | ∼15 | <2 | <2 | >600 |
| Prl-PrP(AV3)11 | 5.7× | ∼15 | <2 | ∼5–8 | ∼200 |
| HuPrP(A117V)36 | 2.4× | ∼15 | ∼4 | NDe | ∼560 |
| Opn-(A117V)33 | 4.0× | ∼10 | <2 | NDe | >700 |
| Transgenea | Expression levelb | CtmPrP in vitroc | CtmPrP in cells | CtmPrP in vivod | Time to disease |
| % | % | d | |||
| PrP(A3922) | 4.0× | ∼10 | <2 | <2 | >700 |
| PrP(KH-II)H | 3.0× | ∼30 | ∼10 | ∼10–20 | ∼60 |
| PrP(KH-II)M | 0.6× | ∼30 | ∼10 | ∼5–8 | ∼470 |
| PrP(A117V)H | 4.0× | ∼15 | ∼4 | ∼5–8 | ∼570 |
| PrP(AV3) | 1–4× | ∼35 | ∼10 | ∼10–20 | <60 |
| Prl-PrP(AV3)6 | 4.7× | ∼15 | <2 | 2–5 | >600 |
| Prl-PrP(AV3)10 | 2.4× | ∼15 | <2 | <2 | >600 |
| Prl-PrP(AV3)11 | 5.7× | ∼15 | <2 | ∼5–8 | ∼200 |
| HuPrP(A117V)36 | 2.4× | ∼15 | ∼4 | NDe | ∼560 |
| Opn-(A117V)33 | 4.0× | ∼10 | <2 | NDe | >700 |
The first five transgenic lines have been described previously (Hegde et al., 1998a, 1999).
Relative to PrP levels in normal hamster (defined as 1×).
Percentage of total PrP from a typical experiment. Exact amounts vary depending on experimental conditions and variations in the translation extract and ER microsomes. However, the relative relationships remain constant.
Arbitrary units; determined by comparing the amount of the diagnostic 18-kD band generated by limited PK digestion to a serial dilution of total brain homogenate.
Indicates not determined; the precise amounts could not be quantified because human PrP seems to behave slightly differently than rodent PrP in the limited PK digestion assay.