Sedimentation velocity AUC
| Construct | Predicted molecular mass | Observed molecular mass | Stoichiometry | Sedimentation coefficient | Frictional coefficient (f/f0) |
| kD | kD | S | |||
| MIS12CNSL1-258 | 116.9 | 115.1 | 1:1:1:1 | 4.08 | 2.079 |
| MIS12CNSL1-258 + SPC2457–197–SPC2570–224 | 151.3 | 160 | 1:1:1:1:1:1 | 4.65 | 2.26 |
| MIS12CNSL1-258 + HP1 | 161.3 | 158 | 1:1:1:1:2 | 4.84 | 2.17 |
| SPC2457–197–SPC2570–224 | 34.4 | 36 | 1:1 | 2.46 | 1.59 |
| HP1 | 44.4 | 45.7 | Homodimer | 2.69 | 1.7 |
| Construct | Predicted molecular mass | Observed molecular mass | Stoichiometry | Sedimentation coefficient | Frictional coefficient (f/f0) |
| MIS12CNSL1-258 | 116.9 | 115.1 | 1:1:1:1 | 4.08 | 2.079 |
| MIS12CNSL1-258 + SPC2457–197–SPC2570–224 | 151.3 | 160 | 1:1:1:1:1:1 | 4.65 | 2.26 |
| MIS12CNSL1-258 + HP1 | 161.3 | 158 | 1:1:1:1:2 | 4.84 | 2.17 |
| SPC2457–197–SPC2570–224 | 34.4 | 36 | 1:1 | 2.46 | 1.59 |
| HP1 | 44.4 | 45.7 | Homodimer | 2.69 | 1.7 |