Table I.

Sedimentation velocity AUC

Construct Predicted molecular mass Observed molecular mass Stoichiometry Sedimentation coefficient Frictional coefficient (f/f0) 
 kD kD  S  
MIS12CNSL1-258 116.9 115.1 1:1:1:1 4.08 2.079 
MIS12CNSL1-258 + SPC2457–197–SPC2570–224 151.3 160 1:1:1:1:1:1 4.65 2.26 
MIS12CNSL1-258 + HP1 161.3 158 1:1:1:1:2 4.84 2.17 
SPC2457–197–SPC2570–224 34.4 36 1:1 2.46 1.59 
HP1 44.4 45.7 Homodimer 2.69 1.7 
Construct Predicted molecular mass Observed molecular mass Stoichiometry Sedimentation coefficient Frictional coefficient (f/f0) 
 kD kD  S  
MIS12CNSL1-258 116.9 115.1 1:1:1:1 4.08 2.079 
MIS12CNSL1-258 + SPC2457–197–SPC2570–224 151.3 160 1:1:1:1:1:1 4.65 2.26 
MIS12CNSL1-258 + HP1 161.3 158 1:1:1:1:2 4.84 2.17 
SPC2457–197–SPC2570–224 34.4 36 1:1 2.46 1.59 
HP1 44.4 45.7 Homodimer 2.69 1.7 
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