Lectin resistance of CHO cells stably expressing GnT1IP-L or -S
| Construct transfected | Selection methoda | L-PHA | WGA |
| GnT1IP-L | Hygro | >10Rb | ndc |
| GnT1IP-L | GNA-sorted | >10R | nd |
| Tag-GnT1IP-L | Hygro | >10R | 7R |
| Tag-GnT1IP-L | GNA-sorted | >10R | nd |
| GnT1IP-L-Tag | Hygro | >10R | nd |
| GnT1IP-L-iTag1-KDNYY | Hygro | >10R | nd |
| Tag-Δstem-GnT1IP-L | Hygro | –d | nd |
| GnT1IP-S | G418 | – | – |
| GnT1IP-S-KDEL | Hygro | – | – |
| Tag-GnT1IP-S | G418 or Hygro | >10R | 3R |
| Tag-GnT1IP-S | GFP-sorted | >10R | >8R |
| Tag-GnT1IP-S (E263A or D265A) | G418 | >10R | 3R |
| GnT1IP-S-Tag | Hygro | – | – |
| SP-iTag2-GnT1IP-S | G418 or Hygro | – | – |
| Tag-Δstem-GnT1IP-S | Hygro | – | – |
| Tag-GnT1IP-S-CD1 | G418 or Hygro | – | – |
| Tag-GnT1IP-S-CD2 | G418 or Hygro | – | – |
| CHO untransfected | 5e | 2e | |
| CHO/vector or GFP | G418 or Hygro | – | – |
| Lec1 untransfected | >10R | >8R |
| Construct transfected | Selection methoda | L-PHA | WGA |
| GnT1IP-L | Hygro | >10Rb | ndc |
| GnT1IP-L | GNA-sorted | >10R | nd |
| Tag-GnT1IP-L | Hygro | >10R | 7R |
| Tag-GnT1IP-L | GNA-sorted | >10R | nd |
| GnT1IP-L-Tag | Hygro | >10R | nd |
| GnT1IP-L-iTag1-KDNYY | Hygro | >10R | nd |
| Tag-Δstem-GnT1IP-L | Hygro | –d | nd |
| GnT1IP-S | G418 | – | – |
| GnT1IP-S-KDEL | Hygro | – | – |
| Tag-GnT1IP-S | G418 or Hygro | >10R | 3R |
| Tag-GnT1IP-S | GFP-sorted | >10R | >8R |
| Tag-GnT1IP-S (E263A or D265A) | G418 | >10R | 3R |
| GnT1IP-S-Tag | Hygro | – | – |
| SP-iTag2-GnT1IP-S | G418 or Hygro | – | – |
| Tag-Δstem-GnT1IP-S | Hygro | – | – |
| Tag-GnT1IP-S-CD1 | G418 or Hygro | – | – |
| Tag-GnT1IP-S-CD2 | G418 or Hygro | – | – |
| CHO untransfected | 5e | 2e | |
| CHO/vector or GFP | G418 or Hygro | – | – |
| Lec1 untransfected | >10R | >8R |
Transfectants were selected for stable expression of constructs in hygromycin (Hygro) or G418 or sorted by flow cytometer for binding of GNA.
Number represents fold-change in lectin resistance from CHO; R, resistant.
nd, not determined.
–, no change in lectin resistance compared to CHO untransfected.
Concentration of lectin in μg/ml that kills 90% CHO cells.