Progression of microinfected cells through mitosis
Injection . | Cell phase at injection . | Number of cells injected . | Number of cells arrested at prometaphase . | Number of cells arrested at metaphase . | Number of cells that completed mitosis . |
|---|---|---|---|---|---|
| Buffer | Prophase | 8 | 8 | ||
| Prometaphase | 2 | 2 | |||
| Cdc34CA (≥3 mg/mL) | Prophase | 11 | 11 | ||
| Prometaphase | 4 | 4 | |||
| Cdc34 (≥3 mg/mL) | Prophase | 104 | 89 | 5 | 10 |
| Prometaphase | 24 | 18 | 5 | 1 | |
| Late prometaphase | 11 | 4 | 7* | ||
| Anaphase | 6 | 6 | |||
| Cdc34 (3 mg/mL) denatured | Prophase | 6 | 6 | ||
| Prometaphase | 3 | 3 | |||
| Cdc34 (1.5 mg/mL) | Prophase | 11 | 7 | 4 | |
| Cdc34 (3 mg/mL) with 10 μm MG132 or β-lactone | Prophase | 15 | 15 |
Injection . | Cell phase at injection . | Number of cells injected . | Number of cells arrested at prometaphase . | Number of cells arrested at metaphase . | Number of cells that completed mitosis . |
|---|---|---|---|---|---|
| Buffer | Prophase | 8 | 8 | ||
| Prometaphase | 2 | 2 | |||
| Cdc34CA (≥3 mg/mL) | Prophase | 11 | 11 | ||
| Prometaphase | 4 | 4 | |||
| Cdc34 (≥3 mg/mL) | Prophase | 104 | 89 | 5 | 10 |
| Prometaphase | 24 | 18 | 5 | 1 | |
| Late prometaphase | 11 | 4 | 7* | ||
| Anaphase | 6 | 6 | |||
| Cdc34 (3 mg/mL) denatured | Prophase | 6 | 6 | ||
| Prometaphase | 3 | 3 | |||
| Cdc34 (1.5 mg/mL) | Prophase | 11 | 7 | 4 | |
| Cdc34 (3 mg/mL) with 10 μm MG132 or β-lactone | Prophase | 15 | 15 |
These cells spent on average 32.5 min at metaphase, which is significantly different from uninjected cells (P < 0.05).